PMID: 7011939Feb 1, 1981Paper

Polyploidy in islets of normal and diabetic humans

Human Pathology
M N PohlP H Carstens

Abstract

This study was designed as a pilot project to determine whether the increased polyploidization of pancreatic B cells in diabetic mice reported from our laboratory is also characteristic of human diabetes. Nuclei of hematoxylin and eosin stained islets cells were traced by camera lucida and their volumes determined by semiautomatic particle size analysis. Previous studies have confirmed that nuclear volume can be used as an index of polyploidy, since, in mouse and human islets, the nuclear DNA content is directly proportional to the nuclear volume. Five insulin independent and three insulin dependent human diabetic patients and their age and sex matched controls were studied. The percentages of polyploid nuclei in insulin independent diabetic islets were elevated significantly over those in control subjects in four of five cases; the exception was an 85 year old male who was diagnosed as a diabetic only two months prior to death. Of the three insulin dependent diabetics, one, whose islets appeared otherwise normal, had a significantly greater percentage of polyploid nuclei than the controls; the other two, whose islets were markedly hyalinized, exhibited percentages of polyploid nuclei within normal ranges. The implications of r...Continue Reading

Citations

Dec 1, 1990·Cancer·F Graeme-CookC Compton
Jun 1, 1992·Endocrine Pathology·Ingrid Zbieranowski, David Murray
Jan 1, 1987·Pediatric Pathology·R Drut, R M Drut
Jan 1, 1986·Diabetes/metabolism Reviews·G C WeirS Bonner-Weir

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