Polyprenylphosphate-pentoses in mycobacteria are synthesized from 5-phosphoribose pyrophosphate.

The Journal of Biological Chemistry
M S SchermanM R McNeil

Abstract

Polyprenylphosphate-arabinose (in which the polyprenyl unit is found both as decaprenyl and octahydroheptaprenyl) is a donor of mycobacterial cell wall arabinosyl residues. Because of this important role, its biosynthetic pathway, and that of the related lipid, polyprenylphosphate-D-ribose, was investigated. Surprisingly, phosphoribose pyrophosphate was shown to be a key intermediate on the pathway to both polyprenylphosphate-D-pentoses. Thus, incubation of 5-phospho-D-[14C]ribose pyrophosphate with membranes prepared from Mycobacterium smegmatis resulted in the presence of organic-soluble radioactivity that was shown to be, in part, polyprenylphosphate-[14C]arabinose and polyprenylphosphate-[14C]ribose. Two additional intermediates, polyprenylphosphate-5-phospho[14C]ribose and polyprenylphosphate-5-phospho[14C]arabinose, were identified. Further experiments showed that the mature polyprenylphosphate-ribose is formed from phosphoribose pyrophosphate via a two-step pathway involving a transferase to form polyprenylphosphate-5-phosphoribose and then a phosphatase to form the final polyprenylphosphateribose. Polyprenylphosphate-arabinose is formed by a similar pathway with an additional step being the epimerization at C-2 of the r...Continue Reading

References

Sep 1, 1989·Antimicrobial Agents and Chemotherapy·K Takayama, J O Kilburn
Jan 1, 1986·Advances in Carbohydrate Chemistry and Biochemistry·V N Shibaev
Aug 25, 1995·The Journal of Biological Chemistry·B A Wolucka, E de Hoffmann
Dec 1, 1995·Journal of Bacteriology·M SchermanM McNeil

❮ Previous
Next ❯

Citations

Sep 25, 2004·Medicinal Research Reviews·Rama P TripathiVinod K Tiwari
Feb 8, 2002·Archives of Biochemistry and Biophysics·J Stacey KluttsAlan D Elbein
Jan 1, 1999·Bioorganic & Medicinal Chemistry Letters·J D AyersG S Besra
Jan 1, 1997·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·A WestonM R McNeil
Dec 12, 2001·Current Opinion in Chemical Biology·J B Houseknecht, T L Lowary
Jul 17, 1998·Current Opinion in Chemical Biology·D Chatterjee
Jul 5, 2007·Organic & Biomolecular Chemistry·Iain A SmellieAntony J Fairbanks
Jun 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sarah M BattGurdyal S Besra
Aug 6, 2009·Glycobiology·Henrieta SkovierováMary Jackson
May 27, 2005·Antimicrobial Agents and Chemotherapy·Lluis BallellRobert J Young
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Erik C Hett, Eric J Rubin
Mar 17, 2010·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Ping XieQin Zhang
Aug 2, 2002·Expert Opinion on Investigational Drugs·Laurent S Kremer, Gurdyal S Besra
Jan 26, 2010·Medicinal Research Reviews·Francis E UmesiriSteven J Sucheck
Jun 11, 2014·Critical Reviews in Biochemistry and Molecular Biology·Shiva Kumar AngalaMary Jackson
Mar 21, 2002·Expert Opinion on Therapeutic Targets·Shampa KhasnobisDelphi Chatterjee
Apr 24, 2015·ACS Chemical Biology·Miroslav BrecikKatarína Mikušová
Oct 7, 2004·Bioorganic & Medicinal Chemistry·Charla A Centrone, Todd L Lowary
Dec 30, 2016·Microbiology and Molecular Biology Reviews : MMBR·Bjarne Hove-JensenMartin Willemoës
May 20, 2015·Antimicrobial Agents and Chemotherapy·Vadim MakarovStewart T Cole
Sep 16, 2015·Journal of Bacteriology·Gaëlle S KollyStewart T Cole

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.