Polyproline II helical structure in protein unfolded states: lysine peptides revisited

Protein Science : a Publication of the Protein Society
A L Rucker, Trevor P Creamer

Abstract

The left-handed polyproline II (PPII) helix gives rise to a circular dichroism spectrum that is remarkably similar to that of unfolded proteins. This similarity has been used as the basis for the hypothesis that unfolded proteins possess considerable PPII helical content. It has long been known that homopolymers of lysine adopt the PPII helical conformation at neutral pH, presumably a result of electrostatic repulsion between side chains. It is shown here that a seven-residue lysine peptide also adopts the PPII conformation. In contrast with homopolymers of lysine, this short peptide is shown to retain PPII helical character under conditions in which side-chain charges are heavily screened or even neutralized. The most plausible explanation for these observations is that the peptide backbone favors the PPII conformation to maximize favorable interactions with solvent. These data are evidence that unfolded proteins do indeed possess PPII content, indicating that the ensemble of unfolded states is significantly smaller than is commonly assumed.

References

Jan 1, 1991·Annual Review of Biochemistry·K A Dill, D Shortle
Jul 20, 1973·Science·C B Anfinsen
Mar 3, 1970·Biochemistry·W L Mattice, L Mandelkern
Jan 1, 1968·Biopolymers·M L Tiffany, S Krimm
Jan 1, 1968·Biopolymers·M L Tiffany, S Krimm
Feb 18, 1994·Journal of Molecular Biology·K J Lumb, P S Kim
May 1, 1994·Protein Science : a Publication of the Protein Society·A ChakrabarttyR L Baldwin
May 24, 1994·Biochemistry·E E LattmanK A Dill
Jan 20, 1993·Journal of Molecular Biology·A A Adzhubei, M J Sternberg
Mar 7, 1997·Biochimica Et Biophysica Acta·A I ArunkumarC Yu
Aug 1, 1997·Protein Science : a Publication of the Protein Society·S H ParkE Stellwagen
Mar 26, 1999·Protein Science : a Publication of the Protein Society·B J Stapley, T P Creamer
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·R V PappuG D Rose
Nov 29, 2001·Biochemistry·M A KellyT P Creamer

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Citations

Nov 20, 2003·Journal of the American Chemical Society·Stacey E RutledgeAlanna Schepartz
Feb 11, 2009·Nature Biotechnology·Irene NobeliJanet M Thornton
Aug 16, 2012·Nature Communications·Guokui QinDavid L Kaplan
Apr 5, 2007·Nature Protocols·Norma J Greenfield
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Nicholas C Fitzkee, George D Rose
Aug 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Bojan ZagrovicVijay S Pande
Oct 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Haipeng GongGeorge D Rose
Jul 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zhengshuang ShiNeville R Kallenbach
Jun 26, 2008·The Journal of Biological Chemistry·Matthew J FooterJulie A Theriot
Sep 19, 2002·Protein Science : a Publication of the Protein Society·Rohit V Pappu, George D Rose
Nov 14, 2013·PloS One·Amanda P WoonAnna Roujeinikova
Nov 17, 2006·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Sagar D KhareNikolay V Dokholyan
Jan 8, 2013·Journal of Molecular Biology·Atanu Das, Steven S Plotkin
Sep 13, 2016·Nano Letters·Diana YugayPaul S Weiss
Jun 21, 2020·Chemical Biology & Drug Design·Justin Brian V ChiongsonJonel P Saludes
Dec 14, 2017·Beilstein Journal of Organic Chemistry·Vladimir Kubyshkin, Nediljko Budisa
Feb 3, 2021·Journal of Medicinal Chemistry·Armira AzuarMariusz Skwarczynski
Jan 9, 2018·ACS Chemical Neuroscience·George DevittSumeet Mahajan
Sep 16, 2021·Journal of Chemical Theory and Computation·Stéphanie JephthahMarie Skepö
Jul 18, 2021·Protein Science : a Publication of the Protein Society·Sarah F RuidiazBirthe B Kragelund

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