Polypyrrole increases branching and neurite extension by Neuro2A cells on PBAT ultrathin fibers

Nanomedicine : Nanotechnology, Biology, and Medicine
Alessandro E C GranatoMarimelia Porcionatto

Abstract

We present a methodology for production and application of electrospun hybrid materials containing commercial polyester (poly (butylene adipate-co-terephthalate; PBAT), and a conductive polymer (polypyrrole; PPy) as scaffold for neuronal growth and differentiation. The physical-chemical properties of the scaffolds and optimization of the electrospinning parameters are presented. The electrospun scaffolds are biocompatible and allow proper adhesion and spread of mesenchymal stem cells (MSCs). Fibers produced with PBAT with or without PPy were used as scaffold for Neuro2a mouse neuroblastoma cells adhesion and differentiation. Neuro2a adhered to PBAT and PBAT/PPy2% scaffolds without laminin coating. However, Neuro2a failed to differentiate in PBAT when stimulated by treatment with retinoic acid (RA), but differentiated in PBAT/PPy2% fibers. We hypothesize that PBAT hydrophobicity inhibited proper spreading and further differentiation, and inhibition was overcome by coating the PBAT fibers with laminin. We conclude that fibers produced with the combination of PBAT and PPy can support neuronal differentiation.

Citations

Jul 28, 2020·Frontiers in Bioengineering and Biotechnology·Yanru YangZhongze Gu
Jan 10, 2021·International Journal of Molecular Sciences·Kateřina SkopalováPetr Humpolíček
Sep 1, 2020·Tissue & Cell·Alessandro E C GranatoMarimelia Porcionatto
May 25, 2021·ACS Applied Materials & Interfaces·Wevernilson F de DeusBruno V M Rodrigues
Dec 16, 2021·Chemical Reviews·Charalampos PitsalidisRóisín M Owens

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