Polyreactive antigen-binding B cells in the peripheral circulation are IgD+ and B7-

European Journal of Immunology
Z J ChenA L Notkins

Abstract

Polyreactive antibodies are naturally occurring antibodies, primarily of the IgM isotype, that are capable of reacting with a wide variety of different self and non-self antigens. Previously, we reported that a B cell capable of making polyreactive antibody has Ig receptors on its surface that can bind different antigens. The present investigation was initiated to characterize these polyreactive antigen-binding B cells further. A panel of fluorescein isothiocyanate-labeled antigens (insulin, IgG Fc fragment or beta-galactosidase) served as probes to select polyreactive antigen-binding B cells by cell sorting. Our experiment revealed that these polyreactive antigen-binding B cells were mainly of the IgD isotype. They expressed high levels of CD40 and major histocompatibility complex class II molecules, but little or no B7-1, B7-2, or Fas. In contrast to the binding of antigens to monoreactive receptors (usually high affinity), the binding of antigens to polyreactive receptors (usually moderate or low affinity) did not up-regulate the expression of B7-1 or B7-2. Antigens that bound to polyreactive receptors, however, were internalized and degraded, although not as efficiently as antigens that bound to monoreactive receptors. Desp...Continue Reading

References

Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
May 7, 1992·Nature·R J ArmitageC R Maliszewski
Mar 1, 1991·Scandinavian Journal of Immunology·L E MackenzieP M Lydyard
Jan 1, 1992·The Journal of Experimental Medicine·E E Eynon, D C Parker
Jun 15, 1990·Science·R H Schwartz
Jan 1, 1989·Annual Review of Immunology·P Casali, A L Notkins
Jul 1, 1989·The Journal of Cell Biology·H W Davidson, C Watts
Nov 1, 1989·Immunology Today·P Casali, A L Notkins
Jan 1, 1989·Cold Spring Harbor Symposia on Quantitative Biology·R H SchwartzH Quill
Oct 1, 1986·Immunological Reviews·N Gadol, K A Ault
Apr 11, 1985·Nature·A Lanzavecchia
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D H KatzB Benacerraf
Jul 7, 1983·Nature·M V HaspelA L Notkins
Jul 28, 1983·The New England Journal of Medicine·J SatohA L Notkins
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J AnderssonF Melchers
Feb 1, 1995·European Journal of Immunology·Z J ChenA L Notkins
Mar 10, 1995·Science·S Nagata, P Golstein
Dec 1, 1994·Immunological Reviews·P H KrammerM O Westendorp
Jun 1, 1993·Current Opinion in Immunology·S LedermanL Chess
Nov 1, 1994·Journal of Clinical Immunology·G SigounasA L Notkins
Mar 1, 1994·Journal of Clinical Immunology·G SigounasA L Notkins

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Citations

Dec 22, 1999·Clinical and Experimental Immunology·Y J Deng, A L Notkins
Feb 10, 2009·The Journal of Surgical Research·Juyoun LeeJeomil Choi
Aug 5, 2008·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Elizabeth M BradshawJ Christopher Love
Sep 25, 2007·Journal of Autoimmunity·Zhao-Hua ZhouAbner Louis Notkins

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