Polysaccharide-based hydrogels for the immobilization and controlled release of bovine serum albumin

International Journal of Biological Macromolecules
Karine VarnierAlexandre T Paulino

Abstract

Arabic gum-based and chitosan-based hydrogels were synthesized through chemical crosslinking for the immobilization and controlled release of bovine serum albumin (BSA) and characterized by Fourier-transform infrared spectrometry, scanning electron microscopy and swelling assays. The degrees of swelling of the Arabic gum-based hydrogel were 13.22 and 22.95 g water per g dried hydrogel at pH 4.5 and 7.0, respectively, whereas the degrees of swelling of the chitosan-based hydrogel were 15.32 and 36.10 g water per g dried hydrogel, respectively. The water absorption mechanism in both hydrogels was non-Fickian, which involves diffusion through pores and macromolecular relaxation of the hydrophilic three-dimensional polymer network. BSA immobilization capacities of the Arabic gum-based and chitosan-based hydrogels after 240 min at pH 4.5 were 71.0 and 175.6 mg protein per g dried hydrogel, respectively. BSA immobilization capacities after 240 min at pH 7.0 were 62.5 and 154.2 mg protein per g dried hydrogel, respectively. The controlled release of BSA from the Arabic gum-based hydrogel was slightly more efficient than that of the chitosan-based hydrogel due to its more porous structure and weaker physiochemical interactions between ...Continue Reading

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Jul 16, 2017·International Journal of Biological Macromolecules·Fernanda ZonattoAlexandre T Paulino
Apr 15, 2018·International Journal of Biological Macromolecules·Mariane WolfAlexandre Tadeu Paulino

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Citations

Sep 20, 2018·Environmental Science and Pollution Research International·Pâmela Becalli VilelaAlexandre Tadeu Paulino
Oct 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lourdes Amigo, Blanca Hernández-Ledesma
Feb 2, 2020·International Journal of Biological Macromolecules·Mariana Aguiar CargninAlexandre Tadeu Paulino
Jan 7, 2019·International Journal of Biological Macromolecules·Mauricio V CruzKátia F Fernandes

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