Polysaccharide-degrading Enzymes are Unable to Attack Plant Cell Walls without Prior Action by a "Wall-modifying Enzyme".

Plant Physiology
A L Karr, P Albersheim

Abstract

A study of the degradation of plant cell walls by the mixture of enzymes present in Pectinol R-10 is described. A "wall-modifying enzyme" has been purified from this mixture by a combination of diethylaminoethyl cellulose, Bio Gel P-100, and carboxymethyl cellulose chromatography. Treatment of cell walls with the "wall-modifying enzyme" is shown to be a necessary prerequisite to wall degradation catalyzed by a mixture of polysaccharide-degrading enzymes prepared from Pectinol R-10 or by an alpha-galactosidase secreted by the pathogenic fungus Colletotrichum lindemuthianum. The action of the "wall-modifying enzyme" on cell walls is shown to result in both a release of water-soluble, 70% ethanol-insoluble polymers and an alteration of the residual cell wall. A purified preparation of the "wall-modifying enzyme" is unable to degrade a wide variety of polysaccharide, glycoside, and peptide substrates. However, the purified preparation of wall-modifying enzyme has a limited ability to degrade polygalacturonic acid. The fact that polygalacturonic acid inhibits the ability of the "wall-modifying enzyme" to affect cell walls suggests that the "wall-modifying enzyme" may be responsible for the limited polygalacturonic acid-degrading act...Continue Reading

References

Jan 1, 1969·The Journal of General Physiology·M L Evans, P M Ray
Aug 1, 1955·Archives of Biochemistry and Biophysics·M N GREENA M SELIGMAN
Apr 1, 1962·Archives of Biochemistry and Biophysics·P ALBERSHEIM, U KILLIAS
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR
Apr 1, 1955·Biochimica Et Biophysica Acta·G W SCHWERT, Y TAKENAKA
Mar 1, 1960·Plant Physiology·R E Yager
Mar 1, 1962·Plant Physiology·B B Stowe, J B Obreiter
Jul 1, 1967·Plant Physiology·S H LeeR S Bandurski
May 1, 1968·Plant Physiology·A H Datko, G A Maclachlan
Mar 1, 1969·Plant Physiology·F B Abeles

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Citations

Feb 1, 2012·Applied Microbiology and Biotechnology·Anamika RayAndrew J Mort
May 1, 1974·Plant Physiology·F E Sobotka, D A Stelzig
Aug 15, 1992·European Journal of Biochemistry·H J BussinkJ Visser
Jul 28, 2012·Biotechnology for Biofuels·Sayali SaykhedkarAndrew J Mort
Mar 29, 2014·International Journal of Food Microbiology·L VilanovaN Teixidó
Jul 1, 2006·Critical Reviews in Microbiology·Cuixia DiLizhe An
Dec 1, 1979·The Proceedings of the Nutrition Society·I M Morrison
Jul 1, 1977·The Journal of Dermatologic Surgery and Oncology·J Farrant, C A Walter
Jan 22, 1998·Canadian Journal of Microbiology·B PatiñoC Vázquez
Jul 1, 2003·The New Phytologist·Ghislaine RecorbetClaude Alabouvette
Aug 1, 1990·Applied and Environmental Microbiology·J P Keon, G Waksman

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