Polysaccharide FMP-1 from Morchella esculenta attenuates cellular oxidative damage in human alveolar epithelial A549 cells through PI3K/AKT/Nrf2/HO-1 pathway

International Journal of Biological Macromolecules
Wan LiYan Chen

Abstract

Oxidative stress is considered to involve cell death in severe pulmonary diseases like idiopathic pulmonary fibrosis (IPF). Polysaccharide FMP-1 from Morchella esculenta can exert significant antioxidant activity. However, its effects on alveolar epithelial cells remain unperceived. Herein, the effects of FMP-1 against H2O2-induced oxidative damage in human alveolar epithelial A549 cells were investigated. FMP-1 could inhibit H2O2-induced cytochrome C and Caspase-3 release to prevent cell apoptosis via attenuation of MDA and ROS levels, and enhancement the enzymatic activities of SOD and T-AOC. Furthermore, the underlying molecular mechanisms were clarified. The phosphorylation of AKT and the nuclear translocation of Nrf2 were observed to be promoted by FMP-1 as well as the level of HO-1. These findings suggested that FMP-1 attenuate cellular oxidative stress through PI3K/AKT pathway, and FMP-1 could be explored as natural potential antioxidants to lower oxidative stress relevant to the progression of IPF.

References

Feb 21, 2006·Antioxidants & Redox Signaling·Hye-Youn ChoSteven R Kleeberger
Jun 2, 2006·Proceedings of the American Thoracic Society·Moisés Selman, Annie Pardo
Mar 20, 2010·American Journal of Hypertension·Mahmood S MozaffariStephen W Schaffer
Oct 25, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yoon-Jin LeeSang-Han Lee
Nov 5, 2013·Antioxidants & Redox Signaling·Roberto Motterlini, Roberta Foresti
Dec 29, 2013·International Journal of Biological Macromolecules·Li-Hua PanJian-Ping Luo
Apr 22, 2016·Cellular and Molecular Life Sciences : CMLS·Agnieszka LobodaJozef Dulak
Jun 25, 2016·The American Journal of Pathology·Shobha M SwamyVictor J Thannickal
Mar 30, 2017·Critical Reviews in Food Science and Nutrition·Zipora Tietel, Segula Masaphy
Dec 21, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bu-Yan LiaoZhao-Jun Wei

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Citations

Apr 3, 2020·The Journal of International Medical Research·Shiwen KeLiangji Liu
Jan 5, 2020·Bioscience, Biotechnology, and Biochemistry·Zhemin XianGuanghai Yan
Jan 9, 2021·International Journal of Biological Macromolecules· Meng-ZhaoWenjun Wang
Sep 16, 2021·Applied Biochemistry and Biotechnology·Haishan WuLiu Yang

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