Polysaccharide hydrogel combined with mesenchymal stem cells promotes the healing of corneal alkali burn in rats

PloS One
Yifeng KeXiaorong Li

Abstract

Corneal chemical burns are common ophthalmic injuries that may result in permanent visual impairment. Although significant advances have been achieved on the treatment of such cases, the structural and functional restoration of a chemical burn-injured cornea remains challenging. The applications of polysaccharide hydrogel and subconjunctival injection of mesenchymal stem cells (MSCs) have been reported to promote the healing of corneal wounds. In this study, polysaccharide was extracted from Hardy Orchid and mesenchymal stem cells (MSCs) were derived from Sprague-Dawley rats. Supplementation of the polysaccharide significantly enhanced the migration rate of primarily cultured rat corneal epithelial cells. We examined the therapeutic effects of polysaccharide in conjunction with MSCs application on the healing of corneal alkali burns in rats. Compared with either treatment alone, the combination strategy resulted in significantly better recovery of corneal epithelium and reduction in inflammation, neovascularization and opacity of healed cornea. Polysaccharide and MSCs acted additively to increase the expression of anti-inflammatory cytokine (TGF-β), antiangiogenic cytokine (TSP-1) and decrease those promoting inflammation (TNF-...Continue Reading

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Citations

Feb 3, 2016·Biomacromolecules·Zhaoliang ZhangXingyi Li
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Methods Mentioned

BETA
flow cytometry
ELISA
PCR
protein assay
Fluorescence

Software Mentioned

Image Pro Plus
SPSS

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