Polysaccharide PRM3 from Rhynchosia minima root enhances immune function through TLR4-NF-κB pathway

Biochimica Et Biophysica Acta. General Subjects
Xuejing JiaChengwei He

Abstract

Polysaccharides, one of the active ingredients in herbal medicine, are proved to enhance innate immunity against infections. The aim of this study is to explore the immunoregulatory ability of polysaccharides from Rhynchosia minima root in vitro and in vivo. Polysaccharide fractions of R. minima root were obtained by chromatographic column. The content of NO was measured by spectrophotometry. The levels of cytokines (tumor necrosis factor-α, TNF-α; interleukin-6, IL-6; and monocyte chemoattractant protein-1, MCP-1) were determined by enzyme-linked immuno-sorbent assay (ELISA) kits. The translocation of p65 into the nucleus was imaged by confocal microscopy. The mRNA expression of TNF-α, IL-6, and MCP-1 was determined by quantitative real-time PCR. T-lymphocyte subgroups of spleen from immunosuppressive mouse were evaluated by flow cytometry. PRM3 remarkably enhanced the phagocytic ability of macrophages and promoted the release of NO and the secretion of cytokines (TNF-α, IL-6, and MCP-1) from macrophages. Simultaneously, PRM3 potently activated NF-κB signaling pathway via Toll-like receptor 4 (TLR4). In addition, PRM3 obviously increased the levels of serum cytokines, markedly up-regulated the percentages of CD3+ and CD4+ T ly...Continue Reading

Citations

Aug 12, 2018·Nutrients·Tiehua ZhangChanghui Zhao
Jun 28, 2019·International Journal of Biological Macromolecules·Yao WenRongmin Yu
Feb 17, 2021·Bioscience, Biotechnology, and Biochemistry·Fuka TakahashiSachi Tanaka
Mar 10, 2020·Journal of Ethnopharmacology·Barbora NovotnaMaria Cristina Duarte
Apr 6, 2021·Bioactive Materials·Yujun ZengKui Luo
Apr 21, 2021·International Journal of Biological Macromolecules·Yao WenRongmin Yu

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