Polysaccharide templated silver nanowire for ultrasensitive electrical detection of nucleic acids

Analytical Chemistry
Jinming KongNarayanan Balasubramanian

Abstract

An ultrasensitive electrical detection method of nucleic acids has been developed on a nanogapped biosensor. In this study, peptide nucleic acid (PNA) probes were immobilized in the gaps of a pair of finger microelectrodes first and were then hybridized with their complementary target DNA. After that, pectin molecules were introduced into the DNA strand via zirconium-phosphate and zirconium-carbonate chemistries and were oxidated by periodate in acetate buffer (pH 3.98). The newly produced aldedyde groups act as a reactant to reduce ammoniacal silver ion to produce silver nanoparticles, which bridged the gap of the interdigitated microelectrode. The conductance of the metallic nanoparticles correlated directly with the amount of the hybridized DNA. A much higher sensitivity was achieved at 3 femtomolar (S/N > 3) under optimal conditions. This biosensor is also applicable to the direct detection of RNA.

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Citations

Feb 3, 2012·Analytical and Bioanalytical Chemistry·Carlos Briones, Miguel Moreno
Jan 10, 2012·Ultrasonics Sonochemistry·Amir Reza AbbasiAli Morsali
Nov 29, 2011·Journal of Proteomics·Miguel Larguinho, Pedro V Baptista
Apr 2, 2010·Bioelectrochemistry·Ravindra P SinghJeong-Woo Choi
Oct 25, 2016·Ultrasonics Sonochemistry·Kuan-Guan LiuAli Morsali
Jul 10, 2018·International Journal of Analytical Chemistry·Dongxiao WenJinming Kong
Apr 2, 2010·ACS Nano·Daniel A ClaytonShanlin Pan
Nov 16, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Sang Hun Han, Jae-Seung Lee

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