Polysaccharides from astragali radix restore chemical-induced blood vessel loss in zebrafish.

Vascular Cell
Guang HuSimon Ming Yuen Lee

Abstract

Astragali Radix has been used widely for the treatment of cardiovascular and cerebrovascular diseases, and to enhance endurance and stamina in traditional Chinese medicine (TCM) for over 2000 years. The polysaccharide constituents of Astragali Radix (ARP) are considered as one of the major constituents contributing to the multiple pharmacological effects of this medicinal plant. The purpose of the study is to evaluate the vascular regenerative activities of ARPs in a chemically-induced blood vessel loss model in zebrafish. Blood vessel loss was induced in both Tg(fli-1a:EGFP)y1 and Tg(fli-1a:nEGFP)y7 embryos by administration of 300 nM VEGFR tyrosine kinase inhibitor II (VRI) for 3 h at 24 hpf (hour post-fertilization). Then, the blood vessel damaged zebrafish were treated with ARPs for 21 h and 45 h after VRI withdrawal. Morphological changes in intersegmental vessels (ISVs) of zebrafish larvae were observed under the fluorescence microscope and measured quantitatively. The rescue effect of ARPs in the zebrafish models was validated by measuring the relative mRNA expressions of Kdrl, Kdr and Flt-1 using real-time PCR. Two polysaccharide fractions, P4 (50000 D < molecular weight & diameter < 0.1 μm) and P5 (molecular diameter >...Continue Reading

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Citations

Oct 24, 2015·European Journal of Pharmacology·Sai-Wang SetoDennis Chang
Aug 25, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Ming Wai HungSimon Ming Yuen Lee
Jun 9, 2016·Journal of Clinical Medicine·Sai-Wang SetoHosen Kiat
Mar 3, 2020·Frontiers in Pharmacology·Rongchun WangXue Wang
Nov 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nilupaier TayierYuan-Qiang Wang

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Methods Mentioned

BETA
PCR
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Axiovision
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