Polysaccharides from ginseng leaves inhibit tumor metastasis via macrophage and NK cell activation

International Journal of Biological Macromolecules
Myoung-Sook ShinKwang-Soon Shin

Abstract

In this study, we purified the polysaccharide fraction (GS-P) from the leaves of Panax ginseng C.A. Meyer and analyzed its monosaccharide composition and antitumor and antimetastatic activity in vitro and in vivo. GS-P is a 10.2kDa pectic polysaccharide consisting of 15 different monosaccharides. GS-P treatment significantly inhibited metastasis in mice, in a dose-dependent manner. GS-P was not cytotoxic to colon 26-M3.1 cells and increased mouse splenocyte proliferation. Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice. Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells. Pretreatment with anti-asialo GM1 (an antibody for NK cell depletion) partly suppressed the inhibitory effects of GS-P on lung metastasis. These data suggest that GS-P exhibits antimetastatic activity by promoting the activation of macrophages and NK cells.

Citations

Jan 26, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yalin ZhouXin Zhao
Feb 9, 2018·Frontiers in Immunology·Ning MaXi Ma
Aug 23, 2020·Journal of Clinical Medicine·Gustavo R RossiFernando Souza-Fonseca-Guimaraes
Feb 11, 2020·Frontiers in Pharmacology·Dae-Young LeeKwang-Soon Shin
Dec 18, 2020·Food & Function·Mingkun GuoMingxing Wang
Nov 1, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Hanbing LiuXiaohui Fan
Apr 17, 2018·Journal of Agricultural and Food Chemistry·Myoung-Sook ShinKi Sung Kang
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hongyu QiXiangyan Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.