Jan 1, 1981

Polysomal and nonpolysomal messenger RNA of noninduced and induced Friend erythroleukemic cells: analysis by cell-free translation

Differentiation; Research in Biological Diversity
F Gabrielli

Abstract

Polyadenylated and nonpolyadenylated mRNA were prepared from polysomes and from the postribosomal supernatant of noninduced and DMSO-induced Friend cells. The mRNA preparations were translated in a wheat germ cell-free system and the in vitro synthesized proteins, fractionated by polyacrylamide gel electrophoresis, were compared by fluorography. The electrophoretic analysis shows that four preparations of poly (A) + RNA code for many different peptides and that most of these peptides are present in each of the poly (A) + RNA translation products. However, the electrophoretic patterns of these translation products differ in the relative amounts of peptides comigrating in the gel electrophoresis. After DMSO treatment, Friend cells show significative differences in the polysomal and nonpolysomal mRNA pools. With induction, globin becomes the most abundant product of the polysomal poly (A) + RNA, while the relative amounts of peptides coded by nonglobin polysomal poly (A) + RNA are reduced. In parallel, the electrophoretic pattern of the in vitro products on the nonpolysomal poly (A) + RNA changes in the relative amounts of the fractionated peptides; moreover, in induced cells, the nonpolysomal poly (A) + RNA codes for peptides not...Continue Reading

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Mentioned in this Paper

Polyribosomes
Histone antigen
Set of Polysomal Ribosomes
Dimethyl Sulfoxide
SDS-PAGE
Protoplasm
Transcription, Genetic
Protein Biosynthesis
Poly A
Leukemia, Experimental

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