Polysome Fractionation to Analyze mRNA Distribution Profiles

Bio-protocol
Amaresh C PandaMyriam Gorospe

Abstract

Eukaryotic cells adapt to changes in external or internal signals by precisely modulating the expression of specific gene products. The expression of protein-coding genes is controlled at the transcriptional and post-transcriptional levels. Among the latter steps, the regulation of translation is particularly important in cellular processes that require rapid changes in protein expression patterns. The translational efficiency of mRNAs is altered by RNA-binding proteins (RBPs) and noncoding (nc)RNAs such as microRNAs (Panda et al., 2014a and 2014b; Abdelmohsen et al., 2014). The impact of factors that regulate selective mRNA translation is a critical question in RNA biology. Polyribosome (polysome) fractionation analysis is a powerful method to assess the association of ribosomes with a given mRNA. It provides valuable information about the translational status of that mRNA, depending on the number of ribosomes with which they are associated, and identifies mRNAs that are not translated (Panda et al., 2016). mRNAs associated with many ribosomes form large polysomes that are predicted to be actively translated, while mRNAs associated with few or no ribosomes are expected to be translated poorly if at all. In sum, polysome fracti...Continue Reading

References

May 20, 2011·Nature·Björn SchwanhäusserMatthias Selbach
Jun 4, 2014·Molecular and Cellular Biology·Amaresh C PandaMyriam Gorospe
Jul 9, 2014·PloS One·Amaresh C PandaVasudevan Seshadri
Aug 16, 2014·Nucleic Acids Research·Kotb AbdelmohsenMyriam Gorospe
Jan 29, 2016·Nucleic Acids Research·Amaresh C PandaMyriam Gorospe

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Citations

Dec 5, 2018·Scientific Data·Isabela Tiemy PereiraBruno Dallagiovanna
Jan 10, 2019·Nucleic Acids Research·Rodrigo Prado MartinsRobin Fåhraeus
Feb 21, 2019·Cell Cycle·M Laura IddaMyriam Gorospe
Mar 30, 2019·PloS One·Rachel MunkAmaresh C Panda
May 19, 2020·American Journal of Physiology. Endocrinology and Metabolism·Jaclyn E WellesScot R Kimball
Mar 17, 2019·BMC Genomics·Isabela Tiemy PereiraBruno Dallagiovanna
Mar 4, 2020·Cellular & Molecular Biology Letters·Hongda DingChaoliu Dai
Aug 29, 2018·Proceedings of the National Academy of Sciences of the United States of America·Tuna ToptanYuan Chang
Mar 7, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jianbo ChenWei-Shau Hu
Nov 18, 2018·Nature Communications·Bernard KanPascal M Lavoie
Oct 12, 2018·Cancer Discovery·Gnana P KrishnamoorthyJames A Fagin
Aug 23, 2019·Cell Reports·Raquel BujKatherine M Aird
Dec 11, 2020·Npj Aging and Mechanisms of Disease·Andrea Di FrancescoRafael de Cabo
Mar 5, 2021·Bio-protocol·Daniel Shaffer, Jarod A Rollins
Feb 27, 2021·Cancer Research·Kamini SinghHans-Guido Wendel
Jul 17, 2021·Cell & Bioscience·Hongfei XuWeidong Li
Aug 4, 2021·Wiley Interdisciplinary Reviews. RNA·Tanvi SinhaAmaresh Chandra Panda
Oct 29, 2020·ACS Sensors·Mukhil RaveendranPaolo Actis

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