Polystyrene, poly-L-lysine and nylon as adsorptive surfaces for the binding of whole cells of Mycobacterium tuberculosis H37 RV to ELISA plates

Journal of Immunoassay
J A VerschoorK Weyer

Abstract

Several methods of coating whole cells of Mycobacterium tuberculosis H37 RV to ELISA microtitre plates were compared with the aim of developing an ELISA screening assay for murine monoclonal antibodies in culture supernatants and human antibodies in patient sera. Undercoats of nylon or poly-L-lysine were compared to polystyrene as adsorptive surfaces for the bacteria, the effect of increased ionic strength and iclusion of SDS in the coating buffer measured, and methanol (70%) and glutaraldehyde (5%) investigated for their efficiency as fixatives of the bacterial monolayers. The results suggest PBS as a satisfactory coating buffer for the bacterial cells on polystyrene, and 70% methanol the preferred fixative for the dried antigen-coated plates.

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Citations

Mar 1, 1993·Journal of Immunoassay·H Marcotte, M C Lavoie
Jan 21, 2006·Journal of Immunological Methods·N R ThirumalapuraJ R Malayer
Dec 29, 2006·Small·Sergey RozhokRichard C Holz
Dec 13, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Sergey RozhokRichard C Holz

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