Poly(tetrafluoroethylene) separation capillaries for capillary electrophoresis. Properties and applications

Journal of Chromatography. a
Miroslav MackaPaul R Haddad

Abstract

Poly(tetrafluoroethylene) (PTFE) is a material widely known for its inertness and excellent electrical properties. It is also transparent in the UV region and has a reasonable thermal conductivity. These properties make PTFE a suitable material for the separation capillary in capillary electrophoresis. Differences in the chemistry of the capillary wall compared to fused silica (FS) can make PTFE an interesting alternative to FS for some special applications. In this work, properties of a commercial PTFE capillary of approx. 100 microm i.d. were investigated, including the dependence of electroosmotic flow (EOF) on pH for unmodified and dynamically modified PTFE, optical properties, and practical aspects of use. The main problems encountered for the particular PTFE capillary used in this study were that it was mechanically too soft for routine usage and the crystallinity of the PTFE caused light scattering, leading to high background absorbance values in the low UV region. The profile of the EOF versus pH for bare PTFE surprisingly showed significantly negative EOF values at pH < 4.2, with an EOF of -30 x 10(-9) m2 V(-1) s(-1) being observed at pH 2.5. This is likely to be caused by either impurities or additives of basic charac...Continue Reading

References

May 24, 1989·Journal of Chromatography·A Zhu, Y Chen
Jul 15, 1994·Journal of Chromatography. B, Biomedical Applications·J E DickensM Ryan
Oct 25, 2000·Journal of Chromatography. a·K D Altria
Jan 25, 2002·Analytical Chemistry·Miguel A Rodríguez-DelgadoAlejandro Cifuentes
Apr 22, 2004·Lab on a Chip·Andrew de Mello
Oct 31, 2008·Talanta·Holger Becker, Laurie E Locascio

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Citations

Dec 1, 2006·Electrophoresis·Manuel Silva
Mar 15, 2005·Electrophoresis·David C Simpson, Richard D Smith
Sep 7, 2007·Electrophoresis·Pavel CoufalKarel Stulík
Sep 12, 2007·Electrophoresis·Christopher J EvenhuisPaul R Haddad
Feb 16, 2007·Electrophoresis·Fernando BenaventeThomas Hankemeier

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