Polythiophene-g-poly(ethylene glycol) with Lateral Amino Groups as a Novel Matrix for Biosensor Construction

ACS Applied Materials & Interfaces
Huseyin AkbulutYusuf Yagci

Abstract

In the ever-expanding field of conducting polymer research, functionalized graft hybrid copolymers have gained considerable interest in the biomedical engineering and biosensing applications, particularly. In the present work, a new biosensor based on conducting graft copolymer for the detection of phenolic compounds was developed. Thereby, a robust and novel material, namely "polythiophene-g-poly(ethylene glycol) with lateral amino groups" (PT-NH2-g-PEG) hybrid conducting polymer was synthesized via Suzuki condensation polymerization and characterized with (1)H NMR analysis, UV-vis spectroscopy, gel permeation chromatography (GPC) and fluorescence spectroscopy. PT-NH2-g-PEG architecture was then applied as an immobilization matrix to accomplish extended biosensing function. In a typical process, Laccase was utilized as a model enzyme for the detection of phenolic compounds. Detailed surface characterization of PT-NH2-g-PEG/Lac was performed by cyclic voltammetry, electrochemical impedance spectroscopy, atomic force microscopy, fluorescence microscopy and scanning electron microscopy measurements. Optimum pH and polymer amount were found to be pH 6.5 and 0.5 mg polymer, respectively, with the linear range of 0.0025-0.05 mM and ...Continue Reading

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Citations

Oct 1, 2016·Macromolecular Bioscience·Caner GeyikYusuf Yagci
Aug 16, 2017·Polymers·Javad Tavakoli, Youhong Tang
Nov 17, 2020·Analytica Chimica Acta·Paulo A Raymundo-PereiraOrlando Fatibello-Filho
Mar 9, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ya-Qiong ZhangBo Zhu
Feb 1, 2018·ACS Applied Materials & Interfaces·Chen-Jung LeeGang Cheng
Dec 23, 2016·ACS Applied Materials & Interfaces·Sanjay K S PatelJung-Kul Lee
May 24, 2019·ACS Applied Materials & Interfaces·Jhih-Guang WuShyh-Chyang Luo
Oct 6, 2021·Chemical Reviews·Anil KokluSahika Inal

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