Poly(U) and polyadenylation termination signals are interchangeable for terminating the expression of shRNA from a pol II promoter

Biochemical and Biophysical Research Communications
Jun SongRobert Chiu

Abstract

Most short hairpin RNA (shRNA)-expressing vectors use RNA polymerase III promoters, as expression of shRNAs from RNA polymerase II promoters is not well understood, due to the lack of defined transcription initiation sites and functional localization of the transcription termination signal. Here we describe a modified cytomegavirus (CMV) promoter, an RNA polymerase II promoter, to express shRNAs with only four overhangs at the 5' end. The expression of shRNAs from the modified CMV promoter was terminated by the transcription termination, the polyadenylation, or the poly(U) termination signal. These results demonstrated that the poly(U) and polyadenylation termination signals are interchangeable for terminating the expression of shRNAs from the CMV promoter and result in similar efficacies in inhibiting endogenous target genes in mammalian cells.

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Citations

Jul 31, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jeffery C GieringMark A Kay
Jan 9, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Olivier ter BrakeBen Berkhout
Dec 16, 2010·Advanced Drug Delivery Reviews·Feng Li, Ram I Mahato
Apr 13, 2007·Immunology·Chih-Ping MaoT-C Wu
Jan 3, 2006·Journal of Virological Methods·Mei HuangYi-Ping Li
Oct 26, 2010·Genomics, Proteomics & Bioinformatics·Linghu NieYunfeng Feng
Jul 22, 2010·Journal of Pharmacological Sciences·Takashi FujiokaShigehiro Ohdo
Nov 11, 2016·PloS One·Raymond Teck Ho LeePhilip W Ingham

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