Polyunsaturated fatty acid regulation of adipocyte FADS1 and FADS2 expression and function

Obesity
Jessica C RalstonDavid M Mutch

Abstract

Polyunsaturated fatty acids (PUFAs) regulate fatty acid desaturase (FADS1, FADS2) expression in the liver; however, it is unknown whether PUFAs regulate FADS in adipocytes. This is important to study considering reports that link altered desaturase activity with adipose tissue PUFA profiles, body weight, and whole-body glucose homeostasis. Therefore, the present study aimed to determine the direct effects of PUFAs on FADS expression in differentiated 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were treated with either α-linolenic (ALA), linoleic (LA), eicosapentaenoic (EPA), or arachidonic acid (AA). Gene expression, protein abundance, and cellular PUFA content were analyzed by real-time RT-PCR, Western blotting, and gas chromatography, respectively. Fads1 and Fads2 gene expression was reduced by EPA and AA, but not ALA or LA. Reductions in gene expression were reflected in FADS2 protein levels, but not FADS1. Treating cells with ALA and LA led to significant increases in the cellular content of downstream PUFAs. Neither ALA nor EPA changed docosahexaenoic acid content. Differentiated 3T3-L1 adipocytes have a functional FADS pathway that can be regulated by PUFA. Therefore, this common adipocyte model is suitable to stu...Continue Reading

References

Dec 29, 1998·The Journal of Biological Chemistry·H P ChoS D Clarke
Dec 22, 1999·The Journal of Biological Chemistry·H P ChoS D Clarke
Jun 11, 2004·Annual Review of Nutrition·Manabu T Nakamura, Takayuki Y Nara
Sep 14, 2007·Scandinavian Journal of Clinical and Laboratory Investigation·J E SlagsvoldK S Bjerve
Mar 20, 2008·American Journal of Physiology. Endocrinology and Metabolism·Graham P HollowayLawrence L Spriet
Jun 23, 2010·Lipids in Health and Disease·Diana M MerinoDavid M Mutch
Feb 15, 2012·Advances in Nutrition·Nishan S KalupahanaNaima Moustaid-Moussa
Feb 16, 2012·Lipids in Health and Disease·Xiaoping WangYan Gu
Mar 9, 2012·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Holly T ReardonJ Thomas Brenna
Jul 4, 2012·The Journal of Nutritional Biochemistry·Ana Maria LottenbergEdna Regina Nakandakare
Sep 10, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Elizabeth BarberDavid Cameron-Smith
Dec 18, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Ganesan MuraliViswanathan Saraswathi
Feb 26, 2014·International Journal of Obesity : Journal of the International Association for the Study of Obesity·J C RalstonD M Mutch

❮ Previous
Next ❯

Citations

Jan 24, 2016·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Xia QinJ Thomas Brenna
Apr 24, 2018·European Journal of Clinical Investigation·Chia-Yu WangChing-Yi Chen
Aug 8, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Shannon L KlingelDavid M Mutch
Feb 6, 2020·Nutrients·Aleksandra Czumaj, Tomasz Śledziński
Jan 15, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sarthak MatravadiaGraham P Holloway
May 26, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Yan ChenTibor V Varga
Dec 11, 2019·Animals : an Open Access Journal From MDPI·Mingxun LiYongjiang Mao
Aug 23, 2017·Annual Review of Nutrition·Jessica C RalstonHelen M Roche
Jun 15, 2018·Nutrients·Bárbara BraynerKatherine M Livingstone
Mar 24, 2020·The World Journal of Men's Health·Salvatore Chirumbolo
Dec 7, 2019·Archives Animal Breeding·Witold Stanisław ProskuraAndrzej Dybus
Jan 28, 2021·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Anita MorandiClaudio Maffeis
Mar 4, 2020·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Maroua MbarikMarc E Surette
Jun 7, 2020·Current Opinion in Pharmacology·Liam H Brown, David M Mutch
Jun 26, 2021·Tissue & Cell·Lydia SegallaAndrea Sbarbati
Aug 15, 2021·The Science of the Total Environment·Elin SørhusKai K Lie

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.