Polyurethane foam containing rhEGF as a dressing material for healing diabetic wounds: Synthesis, characterization, in vitro and in vivo studies

Colloids and Surfaces. B, Biointerfaces
Do Gi PyunDoo Sung Lee

Abstract

Diabetic wounds are a major health issue associated with diabetes mellitus. To surmount this issue, we developed polyurethane foams (PUFs) incorporating varying amounts of recombinant human epidermal growth factor (rhEGF) (rhEGF-PUFs) as a wound dressing for diabetic wounds. From electron microscopy images, it was found that the pore size of the rhEGF-PUFs surface (the wound contact layer) was less than 100μm, regardless of rhEGF content. The release of rhEGF from the PUFs was evaluated using an enzyme-linked immunosorbent assay. The result showed that the release of rhEGF was time and concentration dependent, i.e., the amount of released rhEGF significantly increased as the immersion time and the rhEGF content of the PUFs increased. In vitro cytotoxicity testing showed that rhEGF-PUFs increased the viability of HaCaT human keratinocytes and CCD986-sk human fibroblasts, which indicated that the incorporated rhEGF maintained its biological activity. In an in vitro scratch wound healing assay, the wound closure rate was faster in CCD986-sk human fibroblasts than in HaCaT human keratinocytes. Finally, the rhEGF-PUFs were evaluated as an in vivo treatment in a full-thickness wound model in diabetized Sprague-Dawley rats. The result...Continue Reading

References

Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Jun 17, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Woo-Ram LeeKwan-Kyu Park
Mar 10, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Thawatchai PhaechamudYasuhiko Tabata

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Citations

Mar 7, 2017·Journal of Pharmaceutical Sciences·Barbara S Gregorí ValdesHelena Margarida Ribeiro
May 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Guixue XiaXiguang Chen
Jun 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sivakumar SingaraveluUma Tiruchirapalli Sivagnanam
Dec 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hye Jin SimDoo Sung Lee
May 10, 2017·Journal of Tissue Engineering and Regenerative Medicine·Ashang Luwang LaivaMichael B Keogh
Dec 19, 2020·Expert Opinion on Drug Delivery·Ovidio CatanzanoJoshua S Boateng
Oct 27, 2020·Bioactive Materials·Sophie Wendels, Luc Avérous
Feb 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Satish PatelDeependra Singh

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