Polyuridylylation and processing of transcripts from multiple gene minicircles in chloroplasts of the dinoflagellate Amphidinium carterae.

Plant Molecular Biology
Adrian C BarbrookChristopher J Howe

Abstract

Although transcription and transcript processing in the chloroplasts of plants have been extensively characterised, the RNA metabolism of other chloroplast lineages across the eukaryotes remains poorly understood. In this paper, we use RT-PCR to study transcription and transcript processing in the chloroplasts of Amphidinium carterae, a model peridinin-containing dinoflagellate. These organisms have a highly unusual chloroplast genome, with genes located on multiple small 'minicircle' elements, and a number of idiosyncratic features of RNA metabolism including transcription via a rolling circle mechanism, and 3' terminal polyuridylylation of transcripts. We demonstrate that transcription occurs in A. carterae via a rolling circle mechanism, as previously shown in the dinoflagellate Heterocapsa, and present evidence for the production of both polycistronic and monocistronic transcripts from A. carterae minicircles, including several regions containing ORFs previously not known to be expressed. We demonstrate the presence of both polyuridylylated and non-polyuridylylated transcripts in A. carterae, and show that polycistronic transcripts can be terminally polyuridylylated. We present a model for RNA metabolism in dinoflagellate c...Continue Reading

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Citations

Nov 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Richard G Dorrell, Christopher J Howe
Jan 24, 2014·PLoS Genetics·Richard G DorrellChristopher J Howe
Dec 10, 2013·Protist·Adrian C BarbrookChristopher J Howe
Jun 2, 2014·Genome Biology and Evolution·Sutada MungpakdeeEiichi Shoguchi
May 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·Richard G Dorrell, Christopher J Howe
Aug 4, 2016·Molecular and Biochemical Parasitology·R E R Nisbet, J L McKenzie
Jul 1, 2016·Annual Review of Microbiology·David Roy Smith, Patrick J Keeling
Dec 29, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jan JanouškovecJuan F Saldarriaga
Apr 5, 2018·Genome Biology and Evolution·Christen M KlingerElisabeth Richardson
Jul 19, 2019·ELife·Isabel C NimmoChristopher J Howe
Jun 3, 2018·Current Genetics·A Bruce Cahoon, Ali A Qureshi
Sep 25, 2017·G3 : Genes - Genomes - Genetics·Matheus Sanitá Lima, David Roy Smith
Sep 30, 2021·Plant Biotechnology Journal·Alessandro OcchialiniScott C Lenaghan

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