Ponicidin inhibits pro-inflammatory cytokine TNF-α-induced epithelial-mesenchymal transition and metastasis of colorectal cancer cells via suppressing the AKT/GSK-3β/Snail pathway

Inflammopharmacology
Zhengguang ZhangCunsi Shen

Abstract

Ponicidin (PON), a natural diterpenoid compound, has been shown to exhibit potent anticancer activities in a wide variety of cancers, including colorectal cancer (CRC). Nevertheless, the precise mechanisms underlying the anti-metastasis effect of PON have not yet been completely defined. The present study was designed to uncover the inhibitory effect of PON on epithelial-mesenchymal transition (EMT), migration and invasion of HCT116 cells induced by pro-inflammatory cytokine tumor necrosis factor-α (TNF-α) in vitro, and liver metastasis in vivo. Briefly, cell proliferation was assessed by Cell Counting Kit-8 assay, followed by wound healing and transwell assays to evaluate cell migration and invasion. The EMT-related molecular markers were determined through quantitative real-time polymerase chain reaction (qPCR), immunofluorescence (IF), western blot (WB), and immunohistochemistry (IHC). Additionally, WB was used to assess the expression of AKT, phosphorylated AKT (p-AKT), GSK-3β, and phosphorylated GSK-3β (p-GSK-3β). As a result, PON could effectively suppress EMT, migration, and invasion in HCT116 cells in vitro, and liver metastasis of HCT116 cells in vivo. Additionally, PON administration also dramatically altered the expr...Continue Reading

References

May 8, 2002·Nature Reviews. Molecular Cell Biology·M Angela Nieto
Feb 17, 2005·Digestive Diseases and Sciences·Hemant K RoyRamesh K Wali
Oct 20, 2006·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·J-F ZhangH Li
Jun 23, 2007·The Journal of Surgical Research·Tao YinMing Yang
Mar 31, 2009·International Journal of Molecular Sciences·Jia-Jun LiuRuo-Zhi Xiao
Apr 4, 2009·Nature Reviews. Cancer·Frances Balkwill
Aug 18, 2010·Current Medicinal Chemistry·Loris Bertazza, Simone Mocellin
Aug 23, 2011·Experimental Eye Research·Jeong Goo LeeEunDuck P Kay
Sep 2, 2011·Chinese Journal of Cancer·Yifan Wang, Binhua P Zhou
Oct 31, 2013·Current Cancer Drug Targets·Yifan WangBinhua P Zhou
Apr 25, 2014·Expert Opinion on Therapeutic Targets·Kevin R GinnebaughFazlul H Sarkar
May 17, 2014·World Journal of Gastroenterology : WJG·Zhao PengQiang Tong
May 29, 2014·Journal of Hepato-biliary-pancreatic Sciences·Anchalee TechasenPuangrat Yongvanit
Jan 15, 2015·International Journal of Molecular Sciences·Yuan-Fei LiuWu-Ming Kong
Jun 21, 2015·Pathology, Research and Practice·Hui CaoMaode Lai
Nov 10, 2015·Future Oncology·Seth A Broster, Natasha Kyprianou
Mar 2, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Dec 5, 2017·Euroasian Journal of Hepato-gastroenterology·Alan I Valderrama-TreviñoEduardo E Montalvo-Javé

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resection
nuclear translocation

Software Mentioned

GraphPad Prism
GraphPad
SPSS
Image Lab
QuantStudio
Image J

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.