Pooled polymerase chain reaction to detect Tritrichomonas foetus in beef bulls

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
James A KennedyJane Carman

Abstract

Preputial scraping samples from 305 mixed breed beef bulls were examined for the detection of Tritrichomonas foetus infection. All samples were collected by veterinarians and transported in commercial media to an accredited lab. Upon arrival samples underwent microscopic examination for the presence of Tritrichomonas foetus and were then incubated until 5 days postcollection before final microscopic examination. Culture detected 14 samples with Trichomonad spp.; all were confirmed to be Tritrichomonas foetus by polymerase chain reaction (PCR). After final examination samples were randomly placed in groups of 5 samples; technicians were blinded as to culture results of the individual samples constituting each pool. From each sample within a group, a portion of the fluid sediment was removed and pooled with the other samples of the group to form 61 pools. From each of the formed pools an aliquot was removed for PCR. PCR detected 16 positive pools; an additional 2 positive samples were then identified on individual PCR on samples previously diagnosed as culture negative. Relative to culture, the 95% confidence intervals for sensitivity and specificity of PCR pools to detect Tritrichomonas foetus were 76.8% to 100% (mean value: 100...Continue Reading

References

May 18, 1999·Preventive Veterinary Medicine·D W CowlingW O Johnson
Dec 12, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Robert H BonDurantKaren A Van Hoosear
Dec 28, 2004·Veterinary Parasitology·R A GrahnL A Lyons
Jun 16, 2005·The Veterinary Clinics of North America. Food Animal Practice·Robert H Bondurant
Oct 31, 2006·The Veterinary Clinics of North America. Food Animal Practice·D Owen Rae, John E Crews

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Citations

Oct 7, 2009·Veterinary Parasitology·Florence Huby-ChiltonAlvin A Gajadhar
Apr 5, 2016·The Veterinary Clinics of North America. Food Animal Practice·Jeff D Ondrak
Dec 18, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Lee EffingerIvan Leyva-Baca
Oct 8, 2021·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Silvia D CarliFranciele M Siqueira

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Methods Mentioned

BETA
scrapings
PCR

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