Pooling Pharyngeal, Anorectal, and Urogenital Samples for Screening Asymptomatic Men Who Have Sex with Men for Chlamydia trachomatis and Neisseria gonorrhoeae.

Journal of Clinical Microbiology
Duygu DurukanEric Pf Chow

Abstract

Screening for Chlamydia trachomatis and Neisseria gonorrhoeae at the pharyngeal, urogenital, and anorectal sites is recommended for men who have sex with men (MSM). Combining the three individual-site samples into a single pooled sample could result in significant cost savings, provided there is no significant sensitivity reduction. The aim of this study was to examine the sensitivity of pooled samples for detecting chlamydia and gonorrhea in asymptomatic MSM using a nucleic acid amplification test. Asymptomatic MSM who tested positive for chlamydia or gonorrhoea were invited to participate. Paired samples were obtained from participants prior to administration of treatment. To form the pooled sample, the anorectal swab was agitated in the urine specimen transport tube and then discarded. The pharyngeal swab and 2 ml of urine sample were then added to the tube. The difference in sensitivity between testing of pooled samples and individual-site testing was calculated against an expanded gold standard, where an individual is considered positive if either pooled-sample or individual-site testing returns a positive result. All samples were tested using the Aptima Combo 2 assay. A total of 162 MSM were enrolled in the study. Sensiti...Continue Reading

References

Jan 17, 1974·The New England Journal of Medicine·H H HandsfieldK K Holmes
Feb 7, 2001·Sexually Transmitted Infections·J P GomesM A Catry
Jul 10, 2004·Journal of Clinical Microbiology·Charlotte A GaydosThomas C Quinn
Nov 26, 2009·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Kyle T BernsteinJeffrey D Klausner
May 23, 2013·Sexually Transmitted Infections·M MitchellM Y Chen
Apr 15, 2018·Sexually Transmitted Infections·Hamish MohammedGwenda Hughes

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