Population-based study of diagnostic assays for Borrelia infection: comparison of purified flagella antigen assay (Ideia, Dako Cytomation) and recombinant antigen assay (Liaison, DiaSorin).

BMC Clinical Pathology
Eskild PetersenSvend Ellermann-Eriksen

Abstract

Testing for Borrelia-specific IgM and IgG-antibodies are often performed on a variety of poorly defined symptoms, and isolated IgM results are a frequent finding, which results in diagnostic uncertainty and further testing. We wanted to test the hypothesis that Borrelia-specific assays using recombinant antigens perform differently from assays based on purified flagella antigen. We compared the use of recombinant antigens (LIAISON DiaSorin, Saluggia, Italy) and purified flagella antigen (IDEIA Borrelia, DakoCytomation, Glostrup, Denmark) in the assay for Borrelia-specific IgM and IgG-antibodies. The assays were tested on an unselected population of serum samples submitted from general practice. A total of 357 consecutive samples for analysis of Borrelia IgM and IgG antibodies. Furthermore, we analysed 540 samples for Borrelia-specific IgM or IgG antibodies first by the IDEIA and, if they were positive, the samples were further analysed using the LIAISON assay. To verify the correctness of the patient's serological status, discrepant samples were analysed by line blots (EcoLine, Virotech). In the consecutive series of 357 samples, the IgM assays detected 308 negative and 3 positive samples with concordant results. Compared with ...Continue Reading

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Methods Mentioned

BETA
dot blot
ELISA
dot blots

Software Mentioned

SAS
DAKO Cytomation

Related Concepts