PMID: 9546154Apr 18, 1998Paper

Population dynamics of phenol-degrading bacteria in activated sludge determined by gyrB-targeted quantitative PCR

Applied and Environmental Microbiology
K WatanabeS Harayama

Abstract

A method for quantifying bacterial populations introduced into an activated-sludge microbial community is described. The method involves extraction of DNA from activated sludge, appropriate dilution of the extracted DNA with DNA extracted from nonintroduced activated sludge, PCR amplification of a gyrB gene fragment from the introduced strain with a set of strain-specific primers, and quantification of the electrophoresed PCR product by densitometry. The adequacy of the method was examined by analyzing the population dynamics of two phenol-degrading bacteria, Pseudomonas putida BH and Comamonas sp. strain E6, that had been introduced into phenol-digesting activated sludge. The density of each of the two populations determined by the PCR method immediately after the introduction was consistent with the density estimated from a plate count of the inoculum. This quantitative PCR method revealed different population dynamics for the two strains in the activated sludge under different phenol-loading conditions. The behavior of both of these strains in the activated sludge reflected the growth kinetics of the strains determined in laboratory axenic cultures.

References

Aug 1, 1975·Biotechnology and Bioengineering·R D Yang, A E Humphrey
Dec 1, 1992·Applied and Environmental Microbiology·W E HolbenJ M Tiedje
Mar 1, 1992·Applied and Environmental Microbiology·L E GreerD R Shelton
Jan 1, 1991·Annual Review of Microbiology·R J Steffan, R M Atlas
Jan 1, 1990·Annual Review of Microbiology·G S Sayler, A C Layton
Jan 1, 1990·Advances in Microbial Physiology·S J Assinder, P A Williams
Oct 1, 1989·Applied and Environmental Microbiology·N C McClureJ C Fry
Sep 1, 1987·Microbiological Reviews·D B Roszak, R R Colwell
May 1, 1966·Journal of General Microbiology·R Y StanierM Doudoroff
Apr 1, 1994·Applied and Environmental Microbiology·J O KaJ M Tiedje
Jul 1, 1996·Applied and Environmental Microbiology·G D DiGiovanniN A Sinclair
Jul 1, 1996·Bioscience, Biotechnology, and Biochemistry·M ShimaoS Harayama
Jul 1, 1997·Applied and Environmental Microbiology·V G MathesonL J Forney
Jun 1, 1994·Applied and Environmental Microbiology·N SaraswatT J Smith

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Citations

May 4, 2005·Biodegradation·Paraskevi N Polymenakou, Euripides G Stephanou
Apr 5, 2012·Journal of Biological Engineering·James Winkler, Katy C Kao
Jun 15, 2014·Applied Microbiology and Biotechnology·Karthiga Nagarajan, Kai-Chee Loh
Jun 28, 2014·Environmental Science and Pollution Research International·Congcong ZhaoQian Wang
Jun 21, 2006·Microbiology and Immunology·Hiroki KawabataHaruo Watanabe
Nov 6, 2015·Frontiers in Microbiology·Fatma A A AzizHiroyuki Futamata
May 10, 2002·Environmental Microbiology·Yuki KasaiShigeaki Harayama
May 16, 2002·Journal of Applied Microbiology·L CavalcaV Andreoni
Aug 31, 2000·Applied and Environmental Microbiology·K WatanabeS Harayama
Sep 22, 2009·Antonie van Leeuwenhoek·Simon J McIlroyDaniel Tillett
Sep 12, 2020·Microbiology Resource Announcements·Fatma Azwani Abdul AzizHiroyuki Futamata
Nov 30, 2005·Journal of Applied Microbiology·S Heiss-BlanquetF Monot
Mar 6, 2021·Antonie van Leeuwenhoek·Xiujun SunWensheng Xiang
Oct 20, 2005·Journal of Bioscience and Bioengineering·K Watanabe, P W Baker
Jan 5, 2000·Journal of Clinical Microbiology·H KasaiS Harayama

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