Population heterogeneity of higher-plant mitochondria in structure and function

European Journal of Cell Biology
Hwa DaiK S Chiang

Abstract

Mitochondria of rapidly developing mungbean seedlings were fractionated into four populations: two density classes, each from a 1500S and a 150S pellet. Each of the four populations exhibited cytochrome c oxidase (COX) activity and contained mitochondrial DNA and cardiolipin; plastid and glyoxysome content were found to be relatively low. Five mitochondrial membrane proteins, COXII/III, ATPase alpha/beta and porin, and a matrix enzyme, manganese superoxide dismutase (MnSOD), were detected by immunoblots in all four populations. Another matrix enzyme, pyruvate dehydrogenase was detected only in the two respiratory-competent 1500S populations. The two 150S populations contained a previously unidentified organelle that lacked demonstrable respiratory capability. This organelle, which we have tentatively referred to as "slow-sedimenting (ss-) mitochondrion", was small in size (below light-optics resolution, 70-300nm, majority < or =200nm) and possessed a peculiar looking boundary membrane, ribosomes, and an occasional prominent electron-dense spot. Characteristically, ss-mitochondria were almost always in contact with a filament-aligned membrane-like structure of varying length. Cristae structure, while undetected in small ss-mitoc...Continue Reading

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Citations

Nov 24, 1999·Micron : the International Research and Review Journal for Microscopy·G A Perkins, T G Frey
Aug 24, 2005·Nucleic Acids Research·Hwa DaiKwen-Sheng Chiang
Feb 13, 2001·Plant Physiology·D C LoganC J Leaver
Oct 11, 2011·The Plant Cell·Yih-Shan LoKwen-Sheng Chiang
Apr 15, 2014·Mitochondrion·Simon R LawJames Whelan
Apr 3, 2016·Free Radical Biology & Medicine·Katie L LiberatoreShahryar F Kianian
Jun 13, 2009·Plant & Cell Physiology·Guangkun YinXinming Jing
Apr 12, 2006·The Journal of Biological Chemistry·Chalivendra C SubbaiahMartin M Sachs
Mar 25, 2006·Journal of Experimental Botany·David C Logan
Mar 2, 2017·Proteomes·Malgorzata CzarnaHanna Janska

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