Population-level analysis of Blastocystis subtype prevalence and variation in the human gut microbiota

Gut
Raul Y TitoJeroen Raes

Abstract

Human gut microbiome studies are mainly bacteria- and archaea-oriented, overlooking the presence of single-cell eukaryotes such as Blastocystis, an enteric stramenopiles with worldwide distribution. Here, we surveyed the prevalence and subtype variation of Blastocystis in faecal samples collected as part of the Flemish Gut Flora Project (FGFP), a Western population cohort. We assessed potential links between Blastocystis subtypes and identified microbiota-host covariates and quantified microbiota differentiation relative to subtype abundances. We profiled stool samples from 616 healthy individuals from the FGFP cohort as well as 107 patients with IBD using amplicon sequencing targeting the V4 variable region of the 16S rRNA and 18S rRNA genes. We evaluated associations of Blastocystis, and their subtypes, with host parameters, diversity and composition of bacterial and archaeal communities. Blastocystis prevalence in the non-clinical population cohort was 30% compared with 4% among Flemish patients with IBD. Within the FGFP cohort, out of 69 previously identified gut microbiota covariates, only age was associated with Blastocystis subtype carrier status. In contrast, a strong association between microbiota community composition...Continue Reading

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Apr 16, 2020·International Journal of Molecular Sciences·Mario MatijašićDonatella Verbanac
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Methods Mentioned

BETA
amplicon sequencing
PCR

Clinical Trials Mentioned

NCT02637115

Software Mentioned

BLAST
microbiome R
dbRDA
UPARSE
LotuS
RDP Classifier
linux32
WGCNA
FastTree
LotuS1

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