Population snapshots predict early haematopoietic and erythroid hierarchies

Nature
Betsabeh Khoramian TusiMerav Socolovsky

Abstract

The formation of red blood cells begins with the differentiation of multipotent haematopoietic progenitors. Reconstructing the steps of this differentiation represents a general challenge in stem-cell biology. Here we used single-cell transcriptomics, fate assays and a theory that allows the prediction of cell fates from population snapshots to demonstrate that mouse haematopoietic progenitors differentiate through a continuous, hierarchical structure into seven blood lineages. We uncovered coupling between the erythroid and the basophil or mast cell fates, a global haematopoietic response to erythroid stress and novel growth factor receptors that regulate erythropoiesis. We defined a flow cytometry sorting strategy to purify early stages of erythroid differentiation, completely isolating classically defined burst-forming and colony-forming progenitors. We also found that the cell cycle is progressively remodelled during erythroid development and during a sharp transcriptional switch that ends the colony-forming progenitor stage and activates terminal differentiation. Our work showcases the utility of linking transcriptomic data to predictive fate models, and provides insights into lineage development in vivo.

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Citations

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Datasets Mentioned

BETA
GSE89754

Methods Mentioned

BETA
RNA-seq
FACS
scRNA-Seq
mechanical dissociation
inDrops
inDrops.py
PCA
FCS
PCR
flow cytometry

Software Mentioned

Population Balance Analysis ( PBA )
L Linv
npy R
Bowtie
Image Lab
compute
SPRING
Linv
GSEA
PBA

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