Abstract
The harlequin ladybird Harmonia axyridis has emerged as a model species in the context of invasion biology and possesses an expanded repertoire of antimicrobial peptides (AMPs). Here we measured the expression of 22 AMP genes in adult beetles from native and introduced populations, and from a biocontrol population, allowing us to compare populations differing in terms of invasive performance. Strikingly, we observed population-specific expression profiles for particular AMPs. Following an immune challenge, the genes for Coleoptericin1 (Col1), Coleoptericin-likeB and Defensin1 were induced up to several thousand times more strongly in the invasive populations compared to the native and biocontrol populations. To determine the role of Col1 in pathogen resistance, the corresponding gene was silenced by RNA interference (RNAi), causing higher mortality in beetles subsequently infected with the entomopathogen Pseudomonas entomophila. The RNAi-triggered susceptibility to this pathogen was reversed by the injection of a synthetic Col1 peptide. We show that a native population is more susceptible to P. entomomophila infection than an invasive population. This is the first study demonstrating population-specific differences in the immun...Continue Reading
References
May 9, 2001·Nucleic Acids Research·M W Pfaffl
May 17, 2006·Trends in Ecology & Evolution·Kelly A Lee, Kirk C Klasing
May 16, 2008·Biometrical Journal. Biometrische Zeitschrift·Torsten HothornPeter Westfall
Feb 22, 2011·Current Biology : CB·Benoît FaconArnaud Estoup
Sep 23, 2011·Biology Letters·Christian Rene RöhrichAndreas Vilcinskas
Oct 19, 2011·Molecular Ecology·E LombaertA Estoup
Sep 6, 2012·Evolutionary Applications·Ashraf TayehBenoit Facon
Nov 23, 2012·Proceedings. Biological Sciences·Andreas VilcinskasHeiko Vogel
Mar 8, 2013·Biology Letters·Henrike SchmidtbergAndreas Vilcinskas
May 21, 2013·Science·Andreas VilcinskasHeiko Vogel
Jan 22, 2014·Journal of Evolutionary Biology·E LombaertT Guillemaud
Aug 19, 2014·Insect Science·Andreas VilcinskasHeiko Vogel
Dec 6, 2014·Developmental and Comparative Immunology·Annika BeckertAndreas Vilcinskas
Apr 4, 2015·Proceedings. Biological Sciences·Mohammad RahnamaeianAndreas Vilcinskas
May 11, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Eleftherios MylonakisAndreas Vilcinskas
Oct 4, 2016·Developmental and Comparative Immunology·Heiko VogelAndreas Vilcinskas
Citations
Mar 15, 2019·PLoS Neglected Tropical Diseases·Josina KellershohnSimone Haeberlein
Sep 19, 2019·Insect Molecular Biology·J GegnerA Vilcinskas
Jun 19, 2018·PeerJ·Ruixue RanFanli Meng
Mar 20, 2019·Frontiers in Physiology·Jasmin GegnerAndreas Vilcinskas
Oct 31, 2018·Scientific Reports·Tobias GegnerKwang-Zin Lee
Oct 2, 2020·Microorganisms·Mengze DuJiufeng Wang
Mar 9, 2019·Ecology and Evolution·Henrike SchmidtbergAndreas Vilcinskas
Sep 16, 2021·Microbiology Spectrum·Kayvan EtebariMichael J Furlong