Population structure and virulence analysis of Alternaria carthami isolates of India using ISSR and SSR markers

World Journal of Microbiology & Biotechnology
Garima Anand, Rupam Kapoor

Abstract

Alternaria leaf spot caused by Alternaria carthami is one of the most devastating diseases of safflower. Diversity among 95 isolates of A. carthami was determined using virulence assays, enzyme assays, dominant (ISSR) and co-dominant (SSR) markers. Collections and isolations were made from three major safflower producing states of India. The virulence assays categorised the population into four groups based on level of virulence. Estimation of activities of cell wall degrading enzymes (CWDE) yielded concurrent results to virulence assays with maximum CWDE activities in most virulent group. Eighteen ISSR primers were used and 23 polymorphic microsatellite markers were developed to assess the genetic diversity and determine the population structure of A. carthami. Analysis of ISSR profiles revealed high genetic diversity (Nei's Genetic diversity index; h = 0.36). Microsatellite markers produced a total of 56 alleles with an average of 2.43 alleles per microsatellite marker and Nei's genetic diversity index as h = 0.43. Unweighted Neighbor-joining and population structure analysis using both the marker systems differently arranged the isolates into three clusters. Distance analysis of the marker profiles provided no evidence for g...Continue Reading

References

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Citations

Sep 25, 2019·Archives of Microbiology·Garima Anand, Rupam Kapoor

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Methods Mentioned

BETA
PCR
genotyping

Software Mentioned

ISSR
POPGENE
Adobe Photoshop CS
Open Government Data ( OGD ) Platform
Statistical Package for Social Sciences
XLSTAT
DARwin

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