Population structure of Endomicrobia in single host cells of termite gut flagellates (Trichonympha spp.)

Microbes and Environments
Hao ZhengAndreas Brune

Abstract

The gut microbiota of many phylogenetically lower termites is dominated by the cellulolytic flagellates of the genus Trichonympha, which are consistently associated with bacterial symbionts. In the case of Endomicrobia, an unusual lineage of endosymbionts of the Elusimicrobia phylum that is also present in other gut flagellates, previous studies have documented strict host specificity, leading to the cospeciation of "Candidatus Endomicrobium trichonymphae" with their respective flagellate hosts. However, it currently remains unclear whether one Trichonympha species is capable of harboring more than one Endomicrobia phylotype. In the present study, we selected single Trichonympha cells from the guts of Zootermopsis nevadensis and Reticulitermes santonensis and characterized their Endomicrobia populations based on internal transcribed spacer (ITS) region sequences. We found that each host cell harbored a homogeneous population of symbionts that were specific to their respective host species, but phylogenetically distinct between each host lineage, corroborating cospeciation being caused by vertical inheritance. The experimental design of the present study also allowed for the identification of an unexpectedly large amount of tag-...Continue Reading

References

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Citations

Jul 22, 2015·Annual Review of Microbiology·Andreas Brune, Carsten Dietrich
Nov 20, 2015·Current Opinion in Chemical Biology·Simon M CraggMartin Zimmer
May 31, 2017·Environmental Microbiology Reports·Kazuki IzawaYuichi Hongoh
Jun 28, 2017·Microbes and Environments·Jun Murase
May 31, 2017·Nature Microbiology·Justin R SeymourRoman Stocker
Jan 13, 2018·Environmental Entomology·Stephen J TaerumGillian H Gile
Sep 30, 2016·Microbes and Environments·Yoichi Kamagata, Takashi Narihiro

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Methods Mentioned

BETA
PCR

Software Mentioned

heatmap
UPARSE
RAxML
R
MAFFT
ARB

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