Populations implicated in anaerobic reductive dechlorination of 1,2-dichloropropane in highly enriched bacterial communities

Applied and Environmental Microbiology
Kirsti M Ritalahti, Frank E Löffler

Abstract

1,2-Dichloropropane (1,2-D), a widespread groundwater contaminant, can be reductively dechlorinated to propene by anaerobic bacteria. To shed light on the populations involved in the detoxification process, a comprehensive 16S rRNA gene-based bacterial community analysis of two enrichment cultures derived from geographically distinct locations was performed. Analysis of terminal restriction fragments, amplicons obtained with dechlorinator-specific PCR primers, and enumeration with quantitative real-time PCR as well as screening clone libraries all implied that Dehalococcoides populations were involved in 1,2-D dechlorination in both enrichment cultures. Physiological traits (e.g., dechlorination in the presence of ampicillin and a requirement for hydrogen as the electron donor) supported the involvement of Dehalococcoides populations in the dechlorination process. These findings expand the spectrum of chloroorganic compounds used by Dehalococcoides species as growth-supporting electron acceptors. The combined molecular approach allowed a comparison between different 16S rRNA gene-based approaches for the detection of Dehalococcoides populations.

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Citations

Dec 4, 2012·Applied and Environmental Microbiology·Shuiquan TangLorenz Adrian
Mar 6, 2007·Applied and Environmental Microbiology·Jianzhong HeLisa Alvarez-Cohen
Dec 20, 2011·Applied and Environmental Microbiology·Shandra D Justicia-LeonFrank E Löffler
Mar 7, 2006·Applied and Environmental Microbiology·Youlboong SungFrank E Löffler
Apr 7, 2006·Applied and Environmental Microbiology·Kirsti M RitalahtiFrank E Löffler
Apr 7, 2006·Applied and Environmental Microbiology·Youlboong SungFrank E Löffler
Feb 23, 2011·International Journal of Molecular Sciences·Supatra AreekitKosum Chansiri
Jan 8, 2014·Environmental Science & Technology·Shandra D Justicia-LeonFrank E Löffler
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Jan 20, 2017·International Journal of Systematic and Evolutionary Microbiology·Janina S KoelschbachRainer U Meckenstock
Dec 12, 2018·Microbiology Resource Announcements·Steven A HigginsFrank E Löffler
Mar 15, 2011·International Journal of Systematic and Evolutionary Microbiology·Kirsti M RitalahtiFrank E Löffler
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Apr 19, 2007·Environmental Science & Technology·Lorenz AdrianStephen H Zinder

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