Populus NST/SND orthologs are key regulators of secondary cell wall formation in wood fibers, phloem fibers and xylem ray parenchyma cells

Tree Physiology
Naoki TakataToru Taniguchi

Abstract

Wood fibers form thick secondary cell wall (SCW) in xylem tissues to give mechanical support to trees. NAC SECONDARY WALL THICKENING PROMOTING FACTOR3/SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN 1 (NST3/SND1) and NST1 were identified as master regulators of SCW formation in xylem fiber cells in the model plant Arabidopsis thaliana. In Populus species, four NST/SND orthologs have been conserved and coordinately control SCW formation in wood fibers and phloem fibers. However, it remains to be elucidated whether SCW formation in other xylem cells, such as ray parenchyma cells and vessel elements, is regulated by NST/SND orthologs in poplar. We knocked out all NST/SND genes in hybrid aspen using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 nuclease (Cas9) system and investigated the detailed histological appearance of stem tissues in the knockout mutants. Observation by light microscopy and transmission electron microscopy showed that SCW was severely suppressed in wood fibers, phloem fibers and xylem ray parenchyma cells in the knockout mutants. Although almost all wood fibers lacked SCW, some fiber cells formed thick cell walls. The irregularly cell wall-forming fibers retaine...Continue Reading

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Citations

Sep 22, 2019·International Journal of Molecular Sciences·Libert B TonfackEshchar Mizrachi
Jul 28, 2019·International Journal of Molecular Sciences·Tobias BruegmannMatthias Fladung
Aug 17, 2021·The Plant Journal : for Cell and Molecular Biology·Naoki TakataToru Taniguchi
Aug 5, 2021·Journal of Integrative Plant Biology·Hui LiQuanzi Li
Nov 1, 2021·Plant & Cell Physiology·Kouki YoshidaNobutaka Mitsuda

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