PopZ identifies the new pole, and PodJ identifies the old pole during polar growth in Agrobacterium tumefaciens

Proceedings of the National Academy of Sciences of the United States of America
Romain GrangeonPatricia C Zambryski

Abstract

Agrobacterium tumefaciens elongates by addition of peptidoglycan (PG) only at the pole created by cell division, the growth pole, whereas the opposite pole, the old pole, is inactive for PG synthesis. How Agrobacterium assigns and maintains pole asymmetry is not understood. Here, we investigated whether polar growth is correlated with novel pole-specific localization of proteins implicated in a variety of growth and cell division pathways. The cell cycle of A. tumefaciens was monitored by time-lapse and superresolution microscopy to image the localization of A. tumefaciens homologs of proteins involved in cell division, PG synthesis and pole identity. FtsZ and FtsA accumulate at the growth pole during elongation, and improved imaging reveals FtsZ disappears from the growth pole and accumulates at the midcell before FtsA. The L,D-transpeptidase Atu0845 was detected mainly at the growth pole. A. tumefaciens specific pole-organizing protein (Pop) PopZAt and polar organelle development (Pod) protein PodJAt exhibited dynamic yet distinct behavior. PopZAt was found exclusively at the growing pole and quickly switches to the new growth poles of both siblings immediately after septation. PodJAt is initially at the old pole but then als...Continue Reading

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Citations

Oct 27, 2016·Nature Microbiology·Jie Xiao, Yves F Dufrêne
Nov 29, 2016·Molecular Microbiology·Nayla FrancisXavier De Bolle
Feb 1, 2017·Nature Reviews. Microbiology·Régis HallezPatrick H Viollier
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May 4, 2016·Journal of Bacteriology·James C Anderson-FurgesonPatricia C Zambryski
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Jul 13, 2017·Annual Review of Microbiology·Prahathees J Eswara, Kumaran S Ramamurthi
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Feb 17, 2019·Scientific Reports·Magalí Graciela BialerAngeles Zorreguieta
Oct 8, 2020·Proceedings of the National Academy of Sciences of the United States of America·J S Robalino-EspinosaP Zambryski
Dec 5, 2017·Trends in Microbiology·Matthieu Bergé, Patrick H Viollier
Aug 10, 2016·Current Opinion in Microbiology·Matthew Howell, Pamela Jb Brown

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