Porcine adipose triglyceride lipase complementary deoxyribonucleic acid clone, expression pattern, and regulation by resveratrol

Journal of Animal Science
T ShanZ Xu

Abstract

Adipose triglyceride lipase (ATGL) was recently identified and described as a major novel triglyceride lipase in animals. In this study, we aimed to study the tissue-specific and developmental expression pattern of porcine ATGL (pATGL) and the effect of resveratrol (RES) on expression of pATGL in vitro. The full-length cDNA sequence of pATGL was 1,958 bp (accession no. EF583921), with a 1,458-bp open reading frame encoding a 486-AA protein (the predicted molecular mass of 53.2 kDa, accession no. ABS58651). Comparison of the deduced AA sequence with the bovine, mouse, rat, dog, and human adipose triglyceride lipase showed 87, 84, 83, 81, and 80% similarity, respectively. Furthermore, the pATGL was highly expressed in porcine adipose tissue, to a lesser degree in kidney, heart, and muscle, and least but detectable in brain. In s.c. adipose tissue, pATGL mRNA was low at birth (1 kg of BW) and then increased, reaching a maximal value at 20 kg of BW (approximately 8 wk old; P < 0.01). In peritoneal and omental adipose tissue, the greatest expression of pATGL was observed at 40 kg of BW (approximately 12 wk old). In vitro, exposure of cultured adipocytes to 40 and 80 muM RES for 24 h increased the mRNA levels of pATGL by 95.3% (P < 0...Continue Reading

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Citations

May 11, 2010·Journal of Applied Genetics·Q-H NieX-Q Zhang
Aug 26, 2014·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Philip Chiu-Tsun TangShun-Wan Chan
May 7, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Johanna SamulinHilde Sundvold
Oct 28, 2008·Journal of Lipid Research·Rudolf ZechnerAchim Lass
Jul 9, 2008·Endocrine Reviews·Marc Prentki, S R Murthy Madiraju
Jan 22, 2010·Molecular Biology Reports·Qinghua NieXiquan Zhang
Sep 20, 2011·Journal of Animal Science·T E Weber, B J Kerr
Aug 31, 2010·Journal of Animal Science·T ShanY Wang

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