Porcine and bovine Clostridium difficile ribotype 078 isolates demonstrate similar growth and toxigenic properties

International Microbiology : the Official Journal of the Spanish Society for Microbiology
Łukasz GrześkowiakWilfried Vahjen

Abstract

Clostridioides (C.) difficile are found in cows, pigs and poultry suggesting that this pathogen is present and more importantly animals could act as a reservoir, via food or environment, of human C. difficile infection. Molecular methods together with phenotypical characterisation bring integrated and important tools to describe diversity and nature of bacteria including C. difficile. Moreover, similar or identical C. difficile types are found in different farm animals. This study aimed to phenotypically characterise C. difficile isolates belonging to ribotype 078 and to identify differences such as growth and toxicity between porcine and bovine isolates. C. difficile isolates were assessed for the growth behaviour (turbidimetry), metabolic potential (Biolog AN) and toxin production (ELISA method) in vitro. The concentration of released either toxin A (TcdA) or toxin B (TcdB) varied greatly between the isolates tested; however, it did not differ between the porcine and bovine ribotypes. Also, the TcdA/TcdB ratio of the isolates did not show a difference either. The most common metabolised substrates were pyruvic acid followed by α-ketobutyric acid. The results show that both porcine and bovine C. difficile RT 078 share similar ...Continue Reading

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Citations

Jul 23, 2019·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Łukasz GrześkowiakJürgen Zentek
Nov 11, 2019·Archives of Microbiology·Łukasz GrześkowiakWilfried Vahjen

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Methods Mentioned

BETA
ELISA
PCR-ribotyping

Software Mentioned

SPSS

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