PMID: 6978362Mar 26, 1982Paper

Porcine C1q and the solid-phase immunoassay of human immune complexes

Journal of Immunological Methods
B ZiolaK Penttinen

Abstract

Experiments were undertaken to determine if porcine C1q could replace human C1q in the solid-phase immunoassay of human immune complexes (ICs). Porcine C1q was obtained by a two-cycle precipitation method involving dialysis against chelating agents in low ionic strength buffer. C1q was adsorbed to polystyrene beads and in vivo- or in vitro-formed ICs binding to the solid-phase C1q were detected with 125I-labeled or horseradish peroxidase-conjugated anti-human gamma antibodies. Unfractionated, heat-aggregated human gamma globulin (delta IgG) could be detected at 20 ng/ml when diluted in buffer only. The detection threshold changed to 40-80 ng delta IgG/ml when the assay was run with buffer containing normal human serum diluted 1: 1000 (the serum dilution used for detecting natural ICs). Analysis of systemic lupus erythematosus sera revealed that 60% contained highly significant levels of ICs (binding greater than or equal to 3 S.D. above the mean of controls). Comparison with platelet aggregation test results revealed a highly significant correlation between the two methods (P less than 0.0001), even though each assay detected ICs in several serum specimens negative in the other test. These results demonstrate that porcine C1q c...Continue Reading

References

Dec 1, 1976·Australian and New Zealand Journal of Medicine·B S Andrews, R Penny
Jan 1, 1978·Journal of Immunological Methods·J W Goding
Apr 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·R W Compans, P W Choppin
Jan 1, 1981·Journal of Immunological Methods·D A PohlS T Roodman

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Citations

Feb 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·B DufauxW Hollmann
May 1, 1984·Journal of Reproductive Immunology·A AlanenO Wager
Apr 1, 1990·Annals of the Rheumatic Diseases·M KorpelaA Pasternack
Apr 1, 1992·Annals of the Rheumatic Diseases·A HietaharjuH Frey
Jan 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G Y MinukL R Sutherland
Oct 1, 1986·Clinical Immunology and Immunopathology·A SalmiM Reunanen

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