Porcine circovirus type 2 ORF4 protein binds heavy chain ferritin

Journal of Biosciences
Qizhuang LvYanming Zhang

Abstract

Porcine circovirus type 2 (PCV2) is the primary infectious agent of PCV-associated disease (PCVAD) in swine. ORF4 protein is a newly identified viral protein of PCV2 and is involved in virus-induced apoptosis. However, the molecular mechanisms of ORF4 protein regulation of apoptosis remain unclear, especially given there is no information regarding any cellular partners of the ORF4 protein. Here, we have utilized the yeast two-hybrid assay and identified four host proteins (FHC, SNRPN, COX8A and Lamin C) interacting with the ORF4 protein. Specially, FHC was chosen for further characterization due to its important role in apoptosis. GST pull-down, subcellular co-location and co-immunoprecipitation assays confirmed that the PCV2 ORF4 protein indeed interacted with the heavy-chain ferritin, which is an interesting clue that will allow us to determine the role of the ORF4 protein in apoptosis.

References

Jul 1, 1991·The Veterinary Quarterly·G WensvoortF Wagenaar
Mar 10, 1997·The Journal of Cell Biology·B SodeikA Helenius
May 30, 1997·Biochimica Et Biophysica Acta·L I Grossman, M I Lomax
Apr 4, 1998·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·G M AllanB M Adair
Oct 4, 2000·The Journal of Experimental Medicine·S BrouardM P Soares
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Gregory A Smith, Lynn W Enquist
Dec 31, 2002·Virology·Andrew K Cheung
Aug 12, 2003·Gene·Maik HüttemannLawrence I Grossman
Sep 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P O BerberatM P Soares
Sep 13, 2003·Journal of Virology·Sanna SuikkanenMaija Vihinen-Ranta
Feb 3, 2006·Genes & Development·Carmen IvorraVicente Andrés
Apr 6, 2006·Animal Health Research Reviews·Joaquim SegalésMariano Domingo
Oct 13, 2006·The Journal of General Virology·Sirje TimmuskMikael Berg
Aug 4, 2009·Virus Research·Tim Finsterbusch, Annette Mankertz
Oct 26, 2011·Virus Research·Xinna GeHanchun Yang
Jul 12, 2014·Virus Genes·Qi-Zhuang LvYan-Ming Zhang

❮ Previous
Next ❯

Citations

Apr 21, 2017·Viruses·Joachim Denner, Annette Mankertz
Sep 21, 2018·Frontiers in Microbiology·Yuhong PanAnchun Cheng
Feb 20, 2021·Frontiers in Cellular and Infection Microbiology·Yanting ZhangRenyong Jia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis