May 29, 2020

Porcine Deltacoronavirus nsp5 Cleaves DCP1A to Decrease Its Antiviral Ability

Journal of Virology
Xinyu ZhuShaobo Xiao

Abstract

Porcine deltacoronavirus (PDCoV) is an emerging swine enteropathogenic coronavirus. The nonstructural protein nsp5, also called 3C-like protease, is responsible for processing viral polyprotein precursors in coronavirus (CoV) replication. Previous studies have shown that PDCoV nsp5 cleaves the NF-κB essential modulator and the signal transducer and activator of transcription 2 to disrupt interferon (IFN) production and signaling, respectively. Whether PDCoV nsp5 also cleaves IFN-stimulated genes (ISGs), IFN-induced antiviral effector molecules, remains unclear. In this study, we screened 14 classical ISGs and found that PDCoV nsp5 cleaved the porcine mRNA-decapping enzyme 1a (pDCP1A) through its protease activity. Similar cleavage of endogenous pDCP1A was also observed in PDCoV-infected cells. PDCoV nsp5 cleaved pDCP1A at glutamine 343 (Q343) and the cleaved pDCP1A fragments, pDCP1A1-343 and pDCP1A344-580, could not inhibit PDCoV infection. Mutant pDCP1A-Q343A, which resists nsp5-mediated cleavage, exhibited a stronger ability to inhibit PDCoV infection compared with wild-type pDCP1A. Interestingly, the Q343 cleavage site is highly conserved in DCP1A homologs from other mammalian species. Further analyses demonstrated that nsp5...Continue Reading

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Mentioned in this Paper

Interferon Production
Study
DNA Replication
IFNA1 gene
Cysteine Proteases
MIRN580 microRNA, human
Mutant Proteins
Cytospin-B
Deltacoronavirus
Interferons

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