PMID: 2507373Jul 1, 1989Paper

Porcine endometrial prolactin receptors detected by homologous radioreceptor assay

Molecular and Cellular Endocrinology
K H Young, Fuller W Bazer

Abstract

Heterologous radioreceptor assays, commonly using ovine prolactin, may generate inconsistent results since prolactin (PRL) from one species may be recognized as growth hormone in another. Homologous radioreceptor assays (RRA) are most similar to the in vivo hormone-tissue receptor environment; however, lactogenic homologous RRAs have been reported only for mouse hepatic membranes. In this study, an assay system was developed to investigate homologous binding for porcine PRL in porcine uterine tissue. The pig does not produce a decidual PRL or a placental lactogen; yet, PRL affects uterine physiology during reproductively important events. Optimal binding conditions established for porcine PRL homologous RRA include 150 micrograms membrane, 45,000 cpm labeled porcine PRL and 500 microliters sodium phosphate buffer pH 7.6, incubated at 25 degrees C for 24 h. Binding of porcine PRL tracer is very low (less than 3%); however, when tissue is treated with the chaotropic agent, MgCl2 (4 M), binding increases from 3 to 28%. Dissociation kinetics show a rate of 3.79 X 10(-6)/s initially, and then 1.63 X 10(-6)/s. Competition for labeled PRL on binding sites with unlabeled porcine PRL results in 80% displacement with unlabeled porcine pr...Continue Reading

References

May 1, 1978·Biology of Reproduction·G H WilliamsR Mortel
May 1, 1976·Endocrinology·R RollandJ M Hammond
Jan 1, 1976·International Journal of Peptide and Protein Research·C H Li
Feb 1, 1985·Journal of Dairy Science·B K VonderhaarT M Horn
Jun 1, 1985·Molecular and Cellular Endocrinology·L S Haro, F J Talamantes
Dec 1, 1985·Molecular and Cellular Endocrinology·L S Haro, F J Talamantes
Jan 1, 1986·American Journal of Obstetrics and Gynecology·M A Raabe, J A McCoshen
Jan 1, 1988·Endocrine Research·F E Grissom, G K Littleton
Dec 1, 1987·Endocrinologia Japonica·S Sakai, F Ike
Mar 1, 1987·Molecular and Cellular Endocrinology·A AshkenaziA Gertler
Jun 1, 1986·Journal of Animal Science·R S KensingerR R Kraeling
Dec 30, 1986·Biochemical and Biophysical Research Communications·M Goedert
Mar 1, 1987·Proceedings of the Society for Experimental Biology and Medicine·P BerthonJ Djiane
Jan 1, 1974·Methods in Enzymology·Y Hatefi, W G Hanstein
Aug 25, 1974·FEBS Letters·M Wallis
Dec 1, 1970·Archives of Biochemistry and Biophysics·C H LiY A Pankov
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D I Linzer, D Nathans
May 1, 1984·The Australian & New Zealand Journal of Obstetrics & Gynaecology·D L Healy
Dec 15, 1984·The Biochemical Journal·S SakaiP A Kelly
Jan 1, 1980·Endocrinology·A A van der GugtenH G Friesen
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Jul 30, 1982·Biochemical and Biophysical Research Communications·B Rae-Venter, T L Dao
Feb 1, 1983·Proceedings of the Society for Experimental Biology and Medicine·P A KellyG Leblanc
Jul 1, 1983·Molecular and Cellular Endocrinology·J RoseJ E Oldfield
Jan 16, 1984·European Journal of Biochemistry·K KohmotoF Sakiyama
Jan 1, 1982·Perspectives in Biology and Medicine·C S Nicoll

❮ Previous
Next ❯

Citations

Jun 18, 1990·Molecular and Cellular Endocrinology·K H YoungF W Bazer
Jul 1, 1991·Molecular and Cellular Endocrinology·S S GalosyD M Laird
Sep 11, 2012·Journal of Animal Science and Biotechnology·Fuller W BazerGuoyao Wu
Aug 15, 2006·Domestic Animal Endocrinology·Josephine F TrottRussell C Hovey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.