Abstract
Deoxynivalenol (DON) is one of the major mycotoxic contaminants of grains, which causes reduced weight gain in pigs. The cytotoxicity of DON to porcine hepatocytes was examined in this study. DON was added at the final concentration of 100, 10, 1, 0.1 or 0.01 microg/ml to the medium of primary cultured hepatocytes. Cell death of the hepatocytes was observed in DON 100 and 10 microg/ml groups from 6 h after the addition, and in DON 100, 10, 1 and 0.1 microg/ml groups at 24 h in a dose-dependent manner. The dead hepatocytes showed chromatin condensation and fragmentation of the nuclei, which are considered characteristic morphological changes of apoptosis. The nuclei of the dead hepatocytes were stained positively by the TUNEL method. Lactate dehydrogenase (LDH) release, which is considered leakage from apoptotic hepatocytes into the medium, was apparent at 24 h after DON addition. Increased caspase-3 activity was seen in DON 100, 10 and 1 microg/ml groups. Albumin secretion into the medium was significantly reduced in DON 100, 10 and 1microg/ml groups, moderately in the 0.1 microg/ml group, and slightly in the 0.01 microg/ml group. These results indicate that DON induced apoptosis through the caspase-3 activation pathway and cau...Continue Reading
References
Feb 1, 1988·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·D B PreluskyJ D Miller
Dec 1, 1973·Experimental Cell Research·P O Seglen
Jan 1, 1995·Natural Toxins·Y UenoI Ueno
Dec 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J J PestkaL E King
Jan 1, 1994·Natural Toxins·D B PreluskyH L Trenholm
Jan 1, 1993·Veterinary Research Communications·B BergsjøH J Larsen
Jun 13, 1997·Mutation Research·S KnasmüllerP M Eckl
May 13, 1999·The Journal of Biological Chemistry·V I Shifrin, P Anderson
Mar 11, 2000·Drug Metabolism Reviews·J G HengstlerF Oesch
Oct 2, 2001·Bioscience, Biotechnology, and Biochemistry·M NagaseN Sakato
Mar 8, 2002·Toxicology·Katsuhiro MiuraYuichi Murayama
Sep 10, 2003·Drug Metabolism Reviews·Rolf GebhardtFranz Oesch
Citations
Jun 22, 2014·Environmental Toxicology and Pharmacology·Daotong LiYongjiang Ma
Feb 25, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·H MurataN Shimada
Apr 7, 2011·The Journal of Veterinary Medical Science·Hideo MurataNobuaki Shimada
Jan 25, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yanna MaHaibin Zhang
Oct 13, 2010·Toxicology Letters·Anne-Kathrin DiesingStefan Kahlert
May 30, 2009·Toxicology Letters·Susanne DöllJohanna Fink-Gremmels
Sep 11, 2007·Toxicology·Maika KönigsHans-Ulrich Humpf
Jul 12, 2008·Molecular Nutrition & Food Research·Maika KönigsHans-Ulrich Humpf
Jul 29, 2011·Journal of Animal Physiology and Animal Nutrition·W A AwadJ Zentek
Apr 3, 2016·Environmental Toxicology and Pharmacology·Chao DengXianbo Deng
Oct 8, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M E GouzeP Galtier
Jun 9, 2005·The Journal of Veterinary Medical Science·Hideko TakayamaHideo Murata
Dec 4, 2014·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Juliana R GerezAna Paula F L Bracarense
Jul 6, 2013·Environmental Toxicology and Pharmacology·Daotong LiYongjiang Ma
Jan 1, 2015·Toxicon : Official Journal of the International Society on Toxinology·Lv-Hui SunDe-Sheng Qi
Jan 2, 2015·Environmental Toxicology and Pharmacology·Zhihua RenLiuhong Shen
May 12, 2010·Journal of Veterinary Science·Osamu MikamiShigeru Miyazaki
Sep 19, 2017·Asian-Australasian Journal of Animal Sciences·Shuai HuangJianbo Cheng
Dec 8, 2009·Journal of Animal Science·G Díaz-LlanoR Friendship
Aug 20, 2019·Journal of Animal Physiology and Animal Nutrition·Junmei WangJing Zhang
Dec 24, 2019·Reproduction in Domestic Animals = Zuchthygiene·Min YangXiaodong Zhang
Dec 12, 2018·Archives of Toxicology·Bonnie ValgaerenGunther Antonissen
May 15, 2019·Toxins·Dominika Ewa Habrowska-GórczyńskaAgnieszka Wanda Piastowska-Ciesielska
Apr 5, 2019·Oxidative Medicine and Cellular Longevity·Zhihua RenJunliang Deng
Oct 31, 2020·Clinical and Experimental Pharmacology & Physiology·Fatma J Al-Saeedi
Jan 28, 2021·Toxins·Li CaoXichun Wang
Mar 21, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ana Paula F L BracarenseIsabelle P Oswald
May 14, 2021·Environmental Pollution·Hai JiaZhenlong Wu
Apr 13, 2019·Journal of Agricultural and Food Chemistry·Shuai WangDesheng Qi
Feb 1, 2013·Chemical Research in Toxicology·Maria WeidnerHans-Ulrich Humpf
Apr 24, 2019·Journal of Agricultural and Food Chemistry·Haifei WangWenbin Bao