Porcine IL12A and IL12B gene mapping, variation and evidence of association with lytic complement and blood leucocyte proliferation traits

International Journal of Immunogenetics
K WimmersS Ponsuksili

Abstract

Interleukin-12, a heterodimeric cytokine consisting of glycosylated subunits of 35 and 40 kDa, is a central molecule in controlling innate as well as adaptive immunity. This study was aimed to investigate the role of IL12A and IL12B as candidate genes for immune competence in pigs. The porcine genes were screened for polymorphism and association analysis was carried out by mixed model analysis with parameters of innate immunity, in vitro haemolytic complement activity in the classical and alternative pathways, in vivo complement activation expressed as C3c serum concentration, and blood leucocyte proliferation measured in F2 animals of a pig resource population based on cross of Duroc and Berlin miniature pig (DUMI resource population). A single nucleotide polymorphism (SNP) in the promoter region (C > A) of IL12A was identified. Two SNPs were detected in intron 4 of IL12B at positions 192 (A > G) and 437 (C > T). Significant effects of IL12 genotypes on complement activity traits and mitogen-induced leucocyte proliferation were found. The IL12A and IL12B genes were assigned to chromosome13 and 16, respectively, by using radiation hybrid analysis and genetic mapping in the DUMI resource population. Mapping and association analy...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J C RenauldJ Van Snick
Mar 15, 1987·The Biochemical Journal·A O HamiltonK Whaley
Jan 1, 1995·European Journal of Immunology·S GillessenM K Gately
Apr 1, 1994·The Journal of Clinical Investigation·M ZhangP F Barnes
Oct 1, 1994·European Journal of Immunology·M BetteB Fleischer
Jun 1, 1996·European Journal of Immunology·Y ToneH Waldmann
May 1, 1996·Genome Research·G A RohrerC W Beattie
Jun 1, 1996·Animal Genetics·L J AlexanderC W Beattie
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A PuelD Mouton
Jun 1, 1997·Veterinary Immunology and Immunopathology·D L Foss, M P Murtaugh
Apr 8, 1998·Biochemistry and Molecular Biology International·P Kovács, I Klöting
Jan 29, 2000·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·V PravicaI V Hutchinson
Apr 13, 2000·Veterinary Immunology and Immunopathology·N R ReddyB N Wilkie
Feb 24, 2001·Genes and Immunity·M A HallJ S Lanchbury
Mar 17, 2001·Microbes and Infection·C L Karp, M Wills-Karp
Dec 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bruno GranAbdolmohamad Rostami
Jan 25, 2003·Veterinary Immunology and Immunopathology·A CrawleyB N Wilkie
Jan 31, 2003·Immunogenetics·Klaus WimmersSiriluck Ponsuksili
May 12, 2004·Veterinary Immunology and Immunopathology·Klaus WimmersSiriluck Ponsuksili
Jun 7, 2005·Veterinary Immunology and Immunopathology·Quincy L Carter, Rafael E Curiel
Feb 16, 2006·Veterinary Immunology and Immunopathology·Claudine R RaymondBruce N Wilkie
May 23, 2006·International Journal of Immunogenetics·M B FreidinV P Puzyrev
May 17, 2007·International Journal of Immunogenetics·S StanilovaG Prakova

❮ Previous
Next ❯

Citations

Nov 30, 2016·Scandinavian Journal of Immunology·A Paradowska-GoryckaM Olesinska

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.