Porcine induced pluripotent stem cell-derived osteoblast-like cells prevent glucocorticoid-induced bone loss in Lanyu pigs

PloS One
Yu-Jing LiaoJenn-Rong Yang

Abstract

The application of appropriate animal models and techniques for the study of osteoporosis is important. Lanyu pigs, a local miniature breed, have been widely used in various biomedical studies in Taiwan. This study aimed to induce bone loss in Lanyu pigs and to examine whether porcine induced pluripotent stem cell (piPSC)-derived osteoblast-like cells could recover bone mass of tibiae via local cell transplantation. piPSCs were directed to differentiate into osteoblast-like cells using osteogenic medium, and differentiated cells expressed osteogenic markers and phenotypes. Twenty mature female Lanyu pigs were divided into four groups, including control (C, 1% calcium diet), treatment 1 (T1, ovariectomy + 1% calcium diet), treatment 2 (T2, ovariectomy + 0.5% calcium diet), and treatment 3 (T3, ovariectomy + 0.5% calcium diet + 1 mg/kg of prednisolone) and were subjected to bone loss induction for twelve months. Micro-CT images revealed that the lowest trabecular bone parameters, such as trabecular bone volume, thickness, separation, number, and total porosity, were detected in the T3 group. The lowest proportions of cortical bone in the proximal metaphysis, proximal diaphysis, and distal diaphysis were also found in the T3 group...Continue Reading

References

Oct 1, 1995·Bone·S C MillerW S Jee
Aug 6, 2000·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·P SzulcP D Delmas
Oct 10, 2001·The British Journal of Nutrition·A Creedon, K D Cashman
Jul 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Babul BorahRoger J Phipps
Aug 22, 2003·Cloning and Stem Cells·Virginie SottileJim McWhir
Oct 17, 2003·European Cells & Materials·A S Turner
Feb 20, 2007·Annals of the New York Academy of Sciences·Dimitrios J Hadjidakis, Ioannis I Androulakis
Apr 26, 2007·American Journal of Physiology. Endocrinology and Metabolism·K E Scholz-AhrensC-C Glüer
Jun 19, 2008·The Journal of Sexual Medicine·Neal MehtaMahadevan Rajasekaran
Jun 10, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mary L BouxseinRalph Müller
Aug 12, 2010·Cellular Reprogramming·Premjit ArpornmaeklongPaul H Krebsbach
Nov 3, 2010·Cell Stem Cell·Steven L Teitelbaum
Nov 6, 2012·Clinical and Experimental Medicine·Yi LiuJinxiang Han
Oct 10, 2013·Laboratory animal research·Sang-Woo KimByung-Hwa Hyun
Jan 11, 2014·Current Osteoporosis Reports·Ben AntebiDan Gazit

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Citations

Aug 10, 2019·Applied Biochemistry and Biotechnology·Hadeer A AglanAhmed A Abd-Rabou
Dec 10, 2020·BMC Veterinary Research·Rachel A ScarfoneThomas G Koch
Jun 3, 2021·International Journal of Molecular Sciences·Elise AasebøØystein Bruserud

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Software Mentioned

ImageJ
Statistical Analysis System ( SAS )
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