Porcine islet isolation, cellular composition and secretory response.

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme
N J CrowtherI C Green

Abstract

Porcine islets were isolated by infusion of a warm collagenase solution into whole pancreata followed by static incubation at 37 degrees C for 15 minutes. The pancreata were then chopped into small pieces and the free islets purified by filtration and centrifugation over a ficoll gradient. The insulin:amylase ratio of the islets compared to that in the intact pancreas was determined in 19 pancreata and indicates that the isolated islets were of a high degree of purity. The distribution of insulin, glucagon, somatostatin and pancreatic polypeptide containing cells in pig pancreas sections was compared with that in rat. Porcine islets were much smaller and less well defined than rat islets with infiltration of acinar material even into the islet core. The levels of insulin, glucagon and somatostatin in porcine pancreas and isolated porcine islets were measured using conventional radioimmunoassay techniques. The ratio of these hormones in the pancreas was 105.1:5.8:1 respectively, and in the islets 105.1:0.68:0.087 respectively. Fragmentation of the islets during the isolation may have led to the loss of glucagon and somatostatin-containing cells. Islets cultured overnight and tested with a range of glucose concentrations for one ...Continue Reading

Citations

Jun 23, 2007·Diabetes & Metabolism·D DufraneJ C Henquin
Feb 1, 1995·Metabolism: Clinical and Experimental·A M RabuazzoF Purrello
Aug 19, 2014·Xenotransplantation·Santosh NagarajuDavid K C Cooper
Sep 25, 2008·Transplantation·Denis Dufrane, Pierre Gianello
Sep 4, 2009·Current Opinion in Nephrology and Hypertension·Denis Dufrane, Pierre Gianello
Sep 6, 2000·Cell Transplantation·C TosoP Morel

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