Porcine islet isolation outcome is not affected by the amount and distribution of collagen in the pancreas

Xenotransplantation
D E HillingE Bouwman

Abstract

Variable islet yields in porcine islet isolation may be caused by the collagen substrate within the pancreas. The aim of the present study was to determine the total amount and distribution of collagen within porcine pancreata and their relationship to islet isolation outcome. A total of 64 juvenile and 76 adult porcine pancreata of eight purebred breeds were histologically examined. The amount of collagen was quantitatively assessed in tissue samples stained with Sirius Red. Collagen distribution was semi-quantitatively determined by assessing the presence of collagen in the endocrine-exocrine interface and within the islet, in tissue samples stained with Sirius Red and anti-insulin. Islet isolation was performed in 58 pancreata of the adult group. Total collagen content and islet encapsulation ranged widely in both adult and juvenile pigs. However, the majority of islets in adult and juvenile pigs had no or only a limited collagen capsule. The difference in collagen content between adult and juvenile pigs could not be explained by age. Furthermore, no differences between adult and juvenile pigs were found in islet encapsulation or the amount of intra-islet collagen. In adult pigs, no significant relationships were found betwe...Continue Reading

References

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Citations

Dec 17, 2010·Xenotransplantation·Mårten K J Schneider, Jörg D Seebach
Aug 6, 2014·Journal of Zhejiang University. Science. B·Hai-tao ZhuBo Wang
Aug 28, 2020·Transplant International : Official Journal of the European Society for Organ Transplantation·Raphael P H MeierThierry Berney

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