Porcine reproductive and respiratory syndrome virus induces interleukin-15 through the NF-κB signaling pathway.

Journal of Virology
Yi FuWen-hai Feng

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) mainly infects macrophages/dendritic cells and modulates cytokine expression in these cells. Interleukin-15 (IL-15) is a pleiotropic cytokine involved in wide range of biological activities. It has been shown to be essential for the generation, activation, and proliferation of NK and NKT cells and for the survival and activation of CD8(+) effector and memory T cells. In this study, we discovered that PRRSV infection upregulated IL-15 production at both the mRNA and protein levels in porcine alveolar macrophages (PAMs), blood monocyte-derived macrophages (BMo), and monocyte-derived dendritic cells (DCs). We subsequently demonstrated that the NF-κB signaling pathway was essential for PRRSV infection-induced IL-15 production. First, addition of an NF-κB inhibitor drastically reduced PRRSV infection-induced IL-15 production. We then found that NF-κB was indeed activated upon PRRSV infection, as evidenced by IκB phosphorylation and degradation. Moreover, we revealed an NF-κB binding motif in the cloned porcine IL-15 (pIL-15) promoter, deletion of which abrogated the pIL-15 promoter activity in PRRSV-infected alveolar macrophages. In addition, we demonstrated that PRRSV nucl...Continue Reading

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Citations

Feb 11, 2014·BioMed Research International·Sujit PujhariAlexander N Zakhartchouk
Mar 28, 2017·Veterinary Microbiology·Hanzhong Ke, Dongwan Yoo
Dec 13, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xuegang LuoGaiping Zhang
Jan 31, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qiong ZhangWen-hai Feng
Apr 1, 2014·Temperature : Multidisciplinary Biomedical Journal·Sandra I Rosado MontillaJoshua T Selsby

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