Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 4 Cleaves Porcine DCP1a To Attenuate Its Antiviral Activity

The Journal of Immunology : Official Journal of the American Association of Immunologists
Ran TaoShaobo Xiao

Abstract

As one of the most significant etiological agents in pigs, porcine reproductive and respiratory syndrome virus (PRRSV) has adversely impacted the global swine industry since it was discovered in the 1980s. The mRNA-decapping enzyme 1a (DCP1a), a regulatory factor involved in removing the 5'-methylguanosine cap from eukaryotic mRNA, has recently been identified as an IFN-stimulated gene. However, the role of DCP1a in PRRSV infection is not well understood. In this study, overexpression and knockdown of porcine DCP1a (pDCP1a) showed that pDCP1a affected PRRSV infection. Interestingly, we found that PRRSV infection significantly downregulated pDCP1a expression at the protein level by cleaving pDCP1a. Furthermore, we demonstrated that PRRSV nonstructural protein 4 (nsp4), a 3C-like proteinase, is responsible for pDCP1a cleavage, and the cleaved site is at glutamic acid 238 (E238) of pDCP1a. The mutant pDCP1a-E238A, which cannot be cleaved by nsp4, showed higher anti-PRRSV activity, and the antiviral effects of two cleavage products (pDCP1a1-238 and pDCP1a239-580) were significantly decreased compared with wild type pDCP1a. Unexpectedly, PRRSV infection or overexpression of nsp4 did not cleave monkey DCP1a, and monkey DCP1a showed a...Continue Reading

References

Mar 22, 2000·The Journal of General Virology·J ZiebuhrA E Gorbalenya
Feb 6, 2004·Nature Structural & Molecular Biology·Meipei SheHaiwei Song
Apr 7, 2004·The Journal of Cell Biology·Nicolas CougotBertrand Séraphin
Aug 25, 2004·Virus Research·Shishan YuanKay S Faaberg
Jul 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xiao-Song HeHarry B Greenberg
May 16, 2007·The Journal of Biological Chemistry·Christian SchindlerThomas Decker
Dec 20, 2007·The Journal of General Virology·Richard E Randall, Stephen Goodbourn
Dec 9, 2008·Molecular Cell·Tobias M Franks, Jens Lykke-Andersen
May 26, 2009·Vaccine·Tjeerd G KimmanNorbert Stockhofe-Zurwieden
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Felix TritschlerOliver Weichenrieder
Apr 16, 2010·Current Biology : CB·Luke A J O'Neill, Andrew G Bowie
Oct 22, 2010·Journal of Virology·Jonathan D DoughertyRichard E Lloyd
Dec 14, 2011·Animal Health Research Reviews·Yongming SangFrank Blecha
Feb 14, 2012·Current Opinion in Virology·John W Schoggins, Charles M Rice
Jun 20, 2012·Current Opinion in Virology·Ranjni J ChandRaymond R R Rowland
Sep 15, 2012·Nature Reviews. Immunology·Nisha K Duggal, Michael Emerman
Aug 14, 2013·The Journal of General Virology·Eric J SnijderYing Fang
Feb 22, 2014·Annual Review of Immunology·William M SchneiderCharles M Rice
Apr 10, 2014·Current Opinion in Virology·John W Schoggins
Dec 10, 2015·Annual Review of Animal Biosciences·Joan K LunneyGourapura J Renukaradhya

❮ Previous
Next ❯

Citations

May 29, 2020·Journal of Virology·Xinyu ZhuShaobo Xiao
Jun 30, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wei WenPing Qian

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.