PMID: 11906930Mar 22, 2002Paper

Porcine sperm factor supports activation and development of bovine nuclear transfer embryos

Biology of Reproduction
Jason G KnottJ M Robl

Abstract

A study was undertaken to determine whether injection of porcine sperm factors (pSF), which trigger oscillations in intracellular calcium concentration ([Ca(2+)](i)) in mammalian oocytes, could be used to activate bovine oocytes during nuclear transfer. To date, only combined treatments that induce a monotonic rise in [Ca(2+)](i) and inhibit either phosphorylation or protein synthesis have been utilized in nuclear transfer. Several doses of pSF were tested. Injection of 5 mg/ml pSF triggered [Ca(2+)](i) oscillations that resembled those associated with fertilization with respect to amplitude and periodicity, and as a result, a high percentage of oocytes underwent activation. Furthermore, this concentration of pSF supported in vitro and in vivo development up to 60-90 days of gestation, comparable to development in control nuclear transfer embryos. Nevertheless, neither activation procedure supported development as well as did fertilization. The effectiveness of pSF as an activating agent in bovine oocytes may have been compromised because pSF was unable to support oscillations past 3-5 h postinjection and a second injection was necessary to extend the [Ca(2+)](i) oscillations. Likewise, a single injection of pSF failed to trigg...Continue Reading

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Citations

Feb 16, 2006·Reproduction, Fertility, and Development·Christopher MalcuitRafael A Fissore
Nov 1, 2003·Cloning and Stem Cells·Marina J SansinenaRobert A Godke
Jan 22, 2004·Cloning and Stem Cells·Cesare GalliGiovanna Lazzari
Sep 19, 2003·Biology of Reproduction·Eddie J SullivanPhilippe Collas
Jul 3, 2009·The Journal of Reproduction and Development·Lei-Wen ZhaoFan-Yi Zeng
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Feb 23, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Hao YanYitao Zeng
May 24, 2008·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jing FuYitao Zeng
May 16, 2019·Cellular Reprogramming·Xian-Rong XiongXiang-Dong Zi
Sep 11, 2007·Animal Reproduction Science·S M HosseiniM H Nasr-Esfahani

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